透露宿主-寄生虫关系通过保存的MITE在原生生物和病毒基因组
Francisco Nadal-Molero1, Riccardo Rosselli1, Silvia Garcia-Juan1
1Department of Physiology, Genetics and Microbiology, University of Alicante, Carr. de San Vicente del Raspeig, s/n, 03690 San Vicente del Raspeig, Alicante, Spain.
Nucleic acids research
|October 29, 2024
概括
微型反转重复可转移元素 (MITE) 在 prokaryotic 基因组中广泛存在. 它们的属性分布为将微生物宿主与它们的病毒联系起来提供了一种新的方法,揭示了新的病毒宿主关联.
科学领域:
- 基因组学就是基因组学.
- 分子进化分子进化
- 生物信息学是一种生物信息学.
背景情况:
- 可转移元素 (TE) 对基因组进化至关重要.
- 微型反向重复可转移元件 (MITE) 是在基因间区域中发现的非自主可转移元件.
- MITE的动员取决于外部的转换酶.
研究的目的:
- 调查MITE在 prokaryotic基因组中的流行和分布.
- 探索MITE作为病毒与宿主关联的标记物的潜力.
- 使用MITEs识别新的病毒与宿主关系.
主要方法:
- 在近1700个 prokaryotic 属和183,232个基因组中对MITE进行了大规模分析.
- 集群分析以确定在属和物种层面上的MITE特异性.
- 在细胞 (cMITEs) 和病毒 (vMITEs) 序列中对MITE进行比较分析.
主要成果:
- 在56.5%的 prokaryotic 基因组中发现了 MITEs,发现了超过140万个cMITEs.
- 97.4%的cMITEs显示属特定分布,23%是物种特定的.
- 51,655个cMITEs在病毒序列 (vMITEs) 中具有对应物,导致379个预测的新宿主病毒关联,包括Neisseria和Bacteroidota中的新兴关联.
结论:
- MITEs在 prokaryotic 基因组中广泛分布,并表现出显著的宿主特异性.
- MITEs作为有效的分子标记物,用于建立病毒与宿主关系.
- 这项研究提出MITE作为一种新且强大的方法来识别和验证病毒与宿主之间的关联.
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