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通过使用Phoenix dactylifera和Musa acuminata合成的Enterobacter chuandaensis菌株AEC合成的细菌纤维素的生产和表征
Ashraf Sami Hassan Al-Hasabe1,2, Ahmad Faizal Bin Abdull Razis3, Nadiya Akmal Binti Baharum1
1Department of Cell & Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor, 43400, Malaysia.
Archives of microbiology
|October 29, 2024
概括
这项研究介绍了使用Enterobacter chuandaensis与水果提取物的细菌纤维素 (BC) 生产. 这种新方法产生了高质量的,生物相容的BC,为各种应用提供了可持续的替代方案.
科学领域:
- 生物技术是生物技术.
- 材料科学 材料科学 材料科学
- 微生物学 微生物学
背景情况:
- 细菌纤维素 (BC) 是一种由葡萄糖单体合成的多功能生物聚合物.
- 可持续的BC生产方法对于其广泛应用至关重要.
- 探索新的微生物菌株和具有成本效益的碳来源对于高效的BC合成至关重要.
研究的目的:
- 为了研究使用Enterobacter chuandaensis的细菌纤维素 (BC) 的生产.
- 为了利用树果 (日) 和Musa acuminata (香) 的水果提取物作为BC合成的碳来源.
- 描述产生的BC.的结构性,物理性和生物相容性.
主要方法:
- 由Enterobacter chuandaensis使用枣和香水果提取物生产的细菌纤维素.
- 使用减弱总反射率-里埃变换红外光谱 (ATR-FTIR) 和X射线衍射 (XRD) 进行了表征.
- 细菌菌株的全基因组测序,分子量测定和细胞毒性检测.
主要成果:
- 该研究通过ATR-FTIR和XRD证实了具有特征性的纤维素峰值的BC产量.
- 肠杆菌 (Enterobacter chuandaensis) 产生了2.8g/L BC与日提取物和2.5g/L与香提取物.
- 生产的BC表现出高结晶度 (74.1),纤维素Iα形式,以及出色的生物相容性 (92-100%的细胞活力).
结论:
- 肠杆菌 (Enterobacter chuandaensis) 可以使用水果提取物作为碳来源有效地生产细菌纤维素.
- 合成的BC具有理想的结构和生物相容性特性,可用于潜在的生物医学应用.
- 这项研究提出了一种新的,可持续的BC生产方法,利用易于获得的水果废物.
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