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室内内氨酸绿对视网膜细胞有毒

Jingting Zhang¹, Chaoyang Zhang², Hai Xie³

¹Department of Pharmacy, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200032, China; Department of Ophthalmology, Shanghai General Hospital (Shanghai First People's Hospital), Shanghai Jiao Tong University School of Medicine, Shanghai, 200080, China.

Biochemical and biophysical research communications

|October 29, 2024

概括

印ocyanine绿色 (ICG) 以剂量和时间依赖的方式导致视网膜细胞死亡. 在眼科手术期间建议谨慎,以尽量减少ICG暴露和度,保护视网膜细胞.

科学领域:

眼科医生眼科眼科
细胞生物学细胞生物学
手术创新在外科创新.

背景情况:

印ocyanine绿色 (ICG) 是一个重要的染色物,用于帕斯普拉纳玻璃切除术 (PPV),用于可视化视网膜和内部限制膜.
了解ICG在视网膜组织上的毒理特征对于眼科手术期间的患者安全至关重要.

研究的目的:

研究印ocyanine绿 (ICG) 对各种视网膜细胞系和老鼠视网膜的体外和体内影响.
为了确定ICG度,暴露时间和视网膜细胞活力之间的关系.

主要方法:

视网膜细胞系 (ARPE-19,rMC-1,BV2,HRMECs,R28) 被暴露在不同度的ICG和葡萄糖溶液中.
在体内研究涉及ICG和对照溶液在老鼠眼中的静脉内注射.
使用细胞计数工具-8 (CCK-8) 试验评估细胞活力,并通过TUNEL试验检测出细胞亡.

主要成果:

两种ICG度在体外显著降低了视网膜细胞活力,较高度导致更大的减少.
在体内,ICG注射导致大鼠视网膜中细胞死亡的增加.
仅仅葡萄糖度升高也显示细胞活力略有下降,但ICG是毒性的主要驱动因素.

结论:

暴露于印ocyanine绿色 (ICG) 显然会增加视网膜细胞死亡,无论是体外还是体外.
视网膜细胞死亡的程度与ICG度和暴露时间成正比.
临床实践应包括使用较低的ICG度,并尽量减少眼部手术期间的暴露时间,以减轻潜在的视网膜毒性.

关键词:

细胞灭亡 (apoptosis) 是一种死亡的过程.细胞死亡细胞死亡印度氨酸绿色是绿色的帕斯普拉纳玻璃切除术 (帕斯普拉纳玻璃切除术)视网膜细胞视网膜细胞

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