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免疫组织化学和mRNA表达之间的比较,以识别人类表皮生长因子受体2-低乳腺癌

Ximena Baez-Navarro¹, Mieke R van Bockstal², Angelique van der Made¹

¹From the Department of Pathology, Erasmus Medical Center, Rotterdam, the Netherlands (Baez-Navarro, van der Made, van Deurzen).

Archives of pathology & laboratory medicine

|October 29, 2024

概括

定量逆转录聚合酶连锁反应 (RT-qPCR) 与 HER2 免疫组织化学 (IHC) 强烈一致,用于识别 HER2 低乳腺癌,但存在差异. 需要进行进一步的试验,以确定临床结果的最佳方法.

科学领域:

在瘤学瘤学.
分子诊断学分子诊断
生物标记分析生物标记分析

背景情况:

低HER2的乳腺癌 (BC) 是由于新型抗体-药物结合物的可向子集.
HER2免疫组织化学 (IHC) 是分类的标准,但在识别低HER2病例时具有观察者间的变异性.

研究的目的:

评估定量逆转录聚合酶连锁反应 (RT-qPCR) 作为识别HER2-低BC的客观方法.
为了比较HER2 mRNA表达水平与IHC得分.

主要方法:

从之前的观察者间研究中分析了88个HER2非放大BC针活检.
从微切割的侵入性瘤细胞中提取的mRNA用于RT-qPCR分析.
将HER2 mRNA水平与IHC评分进行比较 (HER2 0/超低与HER2-低).

主要成果:

在HER2分类方面,RT-qPCR和IHC之间发现了显著的一致性.
然而,58.8%的IHC定义的HER2 0/超低病例通过MammaTyper (RT-qPCR) 被归类为HER2低.
观察到不一致的结果,与IHC相比,MammaTyper可能会高估HER2表达.

结论:

RT-qPCR显示与HER2 IHC有很强的一致性,但在HER2-低BC识别中显示了差异.
在MammaTyper测试可能会高估HER2表达相对于IHC.
未来的大规模试验是必要的,以将这些技术与临床结果相关联.

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