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Enterococcus faecium的分子表征临床分离物收容 erm (T) 来自意大利医院

Marzia Cinthi¹, Sonia N Coccitto², Serena Simoni¹

¹Unit of Microbiology, Department of Life and Environmental Sciences, Polytechnic University of Marche, Ancona, Italy.

Current microbiology

|October 30, 2024

概括

在七个临床隔离物中发现了 erm(T) 基因,该基因在肠球菌中赋予了宏类耐药性. 虽然检测到移动元素,但基因却没有被检测到.

科学领域:

微生物学微生物学
遗传学遗传学是一个
抗微生物耐药性抗微生物耐药性

背景情况:

肠球菌是 nosocomial 感染的重要原因.
这种 erm(T) 基因赋予了对宏类,林科萨米德和B链谱素 (MLS_B) 的耐药性.
有限的数据存在于 erm(T) 携带的临床肠球菌.

研究的目的:

在临床肠球菌分离物中选 erm(T) 基因.
描述与 erm 相关的遗传背景和移动元素.
为了评估 erm(T) 基因的可转移性.

主要方法:

查296个肠球菌分离物,使用PCR检测erm (T).
全基因组测序 (WGS) 用于遗传上下文分析.
逆PCR和结合试验用于评估移动性和可转移性.

主要成果:

在296个肠球菌分离物中,有7个对 erm (((T)) 呈阳性.
WGS揭示了位于染色体上的 erm(T) 在ST80 E. faecium. 内的不同遗传环境中.
反向PCR检测到带有erm(T) 的小圆,但结合试验显示转移不成功.

结论:

erm(T) 基因存在于临床肠球菌中,与移动元素相关,但不易通过结合转移.
由于潜在的转移到其他病原体,对肠球菌中erm(T) 介导的红色素耐药性的持续监测至关重要.
了解肠球菌抗生素耐药性的遗传基础对于感染控制至关重要.

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