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Songxiao Zhong¹, Xindi Li¹, Changhao Li¹

¹Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX, USA.

Nature cell biology

|October 30, 2024

概括

甲基转移酶复合体 (MTC) 和微处理器协调RNA的修饰和处理. 微处理器组件SERRATE (SE) 通过类似液体的冷凝物促进MTC活动,揭示了一种新的相分离机制.

科学领域:

分子生物学分子生物学
在RNA生物学,RNA生物学.
生物化学生物化学

背景情况:

甲基转移酶复合体 (MTC) 调解了N6-腺素 (m6A) RNA的修饰.
微处理器复合体对于微RNA生物发生是必不可少的.
在MTC和微处理器之间的交叉调节仍然在很大程度上未被探索.

研究的目的:

研究MTC与微处理器之间的相互作用.
阐明阶段分离在协调RNA修饰和处理中的作用.

主要方法:

分析MTCB子单元的可溶性和活性.
描述SERRATE (SE) 凝结物的特性.
在SE变体中评估m⁶A水平.
研究微处理器对miRNA位置的招募.
检查m⁶在primi-miRNA基质上的一个读器丰富.

主要成果:

MTC B 亚单元形成不溶性冷凝物,由蛋白酶体调节.
SE形成类似液体的冷凝物,增强MTC子单元B的溶解性,稳定性和活性.
破坏MTC相互作用或相位行为的SE变异降低了m6A水平.
MTC将微处理器招募到miRNA位置以进行共转录裂变.
在pri-miRNAs上的A修改招募了m6A读者,保留了处理的微处理器.

结论:

由SE驱动的相分离对于MTC活动和m⁶A修改至关重要.
MTC和微处理器表现出相互调节,协调RNA处理.
这项研究揭示了RNA修饰和处理中相分离的新机制.

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