在非阻塞性阿佐精子症的精子和塞尔托利细胞中代谢物互转化酶的改变:微阵列数据和体分析
Danial Hashemi Karoii1,2, Hamoon Baghaei3, Ali Shakeri Abroudi4
1Department of Cell and Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran.
Scientific reports
|October 30, 2024
概括
这项研究揭示了非阻断性亚精精子症 (NOA) 患者的丸细胞中代谢物互转化酶的基因表达的改变. 这些发现为NOA提供了新的见解.
科学领域:
- 生殖生物学 生殖生物学
- 分子代谢的分子代谢.
- 基因组学就是基因组学.
背景情况:
- 塞尔托利细胞和精子代谢受性类固醇激素和能量恒温的调节.
- 生殖系统的功能与代谢信号和能量状态有关.
- 非阻塞性精症 (NOA) 是一种影响男性生育能力的疾病.
研究的目的:
- 研究NOA患者与对照者的丸细胞中代谢物互转化酶的基因表达模式.
- 了解导致NOA的分子机制.
- 为了确定NOA的潜在治疗点.
主要方法:
- 微阵列和生物信息学分析2912个编码代谢物互转换酶的基因.
- 从NOA患者和正常对照的精子和塞尔托利细胞中分析基因表达.
- 使用STRING和Cytoscape进行功能和分子相互作用预测,途径丰富分析,WGCNA和scRNA-seq.
主要成果:
- 在NOA患者的精子和塞尔托利细胞中观察到代谢物互转化酶的差异性基因表达.
- 精子中的上调基因包括MOXD1,ACAD10,PCYT1A,ARG1,METTL6,GPLD1,MAOA和CYP46A1.1,这些都是精子中的上调基因.
- 确定的关键途径包括G1/S特异转录,精子中的酸和酸代谢,以及塞尔托利细胞中的p53信号和叶酸代谢.
结论:
- 代谢物互转化酶的改变表达有助于NOA的分子机制.
- 已识别的基因表达模式和途径为NOA病变发生提供了新的见解.
- 该研究强调了NOA治疗的潜在治疗目标.
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