与癌症相关的纤维细胞 (CAFs) 通过通过外部体转移SERPINE2 (PN1) 来调节肺癌恶性进展
Yu Chen1, Sihong Zhu1, Ling Yang1
1Department of Respiratory Diseases, The Second Affiliated Hospital of Nanchang University, Nanchang, 330000, China.
Current molecular medicine
|October 30, 2024
概括
与癌症相关的纤维细胞 (CAFs) 通过通过外体通过蛋白酶nexin-1 (PN1) 转移促进肺癌. 这激活了Toll-like和NF-κB通路,增加了瘤的生长和进展.
科学领域:
- 在瘤学瘤学.
- 细胞生物学 细胞生物学
- 癌症研究 癌症研究
背景情况:
- 与癌症相关的纤维细胞 (CAFs) 在瘤微环境中丰富,并与肺癌进展有关.
- 通过CAFs影响肺癌的确切机制仍然不完全理解.
- 确定CAF介导的途径对于开发向性肺癌疗法至关重要.
研究的目的:
- 阐明CAFs在肺癌进展中的作用.
- 通过外基因组研究CAF介导的肺癌促进机制.
- 确定关键的分子参与者和涉及的信号通路.
主要方法:
- 初级CAF和正常纤维细胞 (NFs) 和它们的外体被分离出来.
- 蛋白酶nexin-1 (PN1) 表达在CAF中使用lentivirus进行操纵.
- 生物信息学分析了PN1表达,生存数据和肺癌的途径.
- 在体外 (MTT,Transwell) 和体内 (异种移植) 模型中评估了癌细胞行为和瘤生长.
主要成果:
- 富含PN1的CAF衍生外体增强了肺癌细胞的增殖,迁移和入侵.
- 肺癌组织中PN1表达升高,与患者存活率负相关.
- 在癌细胞中的PN1操纵改变了它们的侵入性和增殖能力.
- 过度表达PN1的外体激活了Toll-like和NF-κB信号通路,促进了瘤的生长.
结论:
- 通过PN1.1的外体转移,CAFs促进肺癌的进展.
- PN1转移激活了Toll-like和NF-κB信号通路.
- 准PN1或这些途径可能为肺癌提供治疗策略.
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