探索裂口腔中不同表达的蛋白质的调节相互作用,由视网素酸诱导
Liyun Chen1,2,3, Aiwei Ma1,2,3, Lewen Jiang4
1Department of Plastic Surgery and Burn Center, Second Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong, China.
Protein and peptide letters
|October 30, 2024
概括
这项研究确定了关键的蛋白质和途径,参与了视网膜酸 (RA) 诱导的胚胎裂 palates. 这些发现突出了裂口发育和病变的潜在生物标志物.
科学领域:
- 发展生物学 发展生物学
- 蛋白质组学是指蛋白质组学.
- 遗传性疾病 遗传性疾病
背景情况:
- 胚胎裂 palates 是一个重要的先天性疾病.
- 网红素酸 (RA) 是一种已知的原体,可以诱导口腔裂.
- 由于RA引起的口腔裂的潜在分子机制尚未完全理解.
研究的目的:
- 为了确定参与视网膜酸 (RA) 诱导的胚胎口腔裂变的新型蛋白质和途径.
- 为了探索RA治疗的胚胎 palatal 组织的proteomic 景观.
- 为了确定裂口口腔的潜在蛋白质生物标志物.
主要方法:
- 在胚胎14.5日 (E14.5) 使用iTRAQ进行蛋白质组分析,来自对照和RA治疗的小鼠胚胎的 palatal 组织.
- 不同蛋白质表达分析,基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 路径丰富分析.
- 使用STRING构建蛋白质与蛋白质相互作用网络,并使用Cytoscape (cytoHubba插件) 识别枢纽蛋白.
主要成果:
- 在RA引起的裂口 palatal 组织中,有196种蛋白质显著上调和149种蛋白质显著下调.
- 丰富分析显示了细胞质翻译,核糖体生物发生,糖解/葡萄糖生成和谷氨代谢的参与.
- 十个枢纽蛋白,包括Eif4a1,Gapdh和Eno1,被确定为RA诱导的口腔裂的潜在关键参与者和生物标志物.
结论:
- 这项研究提供了RA诱导的胚胎口腔裂的全面蛋白质学概况.
- 核糖体生物发生和糖溶解中断与口腔裂的病原性有关.
- 已识别的枢纽蛋白代表了裂口口腔的潜在治疗标和诊断生物标志物.
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