光终身复合成像在扩展显微镜与可调节的捐赠-接受聚合物点
Jie Liu1,2, Zhihe Liu1, Feixue Mi1
1Guangdong Provincial Key Laboratory of Advanced Biomaterials, Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong 518055, China.
Chemical & biomedical imaging
|October 30, 2024
概括
半导体聚合物点 (Pdots) 通过提供可调节的光寿命和高光子输出,使扩展显微镜中的超分辨率成像成为可能. 这一突破克服了传统光终身成像显微镜 (FLIM) 的局限性.
科学领域:
- 纳米技术纳米技术
- 显微镜的使用方法
- 生物物理学的生物物理.
背景情况:
- 光终身成像显微镜 (FLIM) 对于检测生物分子至关重要,但面临着衍射极限.
- 在FLIM中的常规染料具有有限的光子输出,并且难以超越衍射极限.
- 扩展显微镜 (ExM) 提供超高分辨率,但是光子饥饿,对传统的FLIM具有挑战性.
研究的目的:
- 在膨胀显微镜中引入半导体聚合物点 (Pdots) 作为光寿命成像的新型探针.
- 为了利用Pdots的可调节寿命和高光子预算来克服ExM中的FLIM限制.
- 为了证明多重体终身成像能力,以提高亚细胞结构分辨率.
主要方法:
- 通过调整聚合物组成,开发了三种具有平均寿命为0.4至5ns的光Pdots.
- 在扩展显微镜中利用Pdots进行光终身成像.
- 分析生命周期域中的Pdot分布,尽管有光谱重叠.
主要成果:
- 在终身域中解决了明显的Pdot分布,使多重成像成为可能.
- Pdots的高亮度和光子输出促进了在光子缺乏的ExM中成像.
- 亚细胞结构的空间分辨率约为49纳米.
结论:
- 半导体聚合物点 (Pdots) 是扩展显微镜中光寿命成像的有效探针.
- 可调节的Pdots在超分辨率显微镜中克服了衍射极限和光子饥饿的条件.
- 这种方法显示出细胞生物学中多重生命周期成像应用的巨大潜力.
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