树球miRNAs影响植物微生物群
Harriet Middleton1,2, Jessica Ann Dozois2, Cécile Monard1
1Écosystèmes, Biodiversité, Évolution (ECOBIO), Unité mixte de recherche (UMR) 6553, Centre national de la recherche scientifique (CNRS) - Université de Rennes, Campus Beaulieu, 263 Avenue du Général Leclerc, Rennes, 35042, France.
ISME communications
|October 30, 2024
概括
植物微RNA (miRNA) 存在于树叶球中,可以改变细菌基因表达和微生物社区组成. 这些发现表明植物miRNAs是土壤微生物群的关键调节者.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 植物科学 植物科学
背景情况:
- 小核糖核酸 (RNAs),包括微核糖核酸 (miRNAs),对于跨王国通信至关重要.
- 已检测到植物miRNAs调节肠道微生物群中的基因表达,并可能影响根球微生物.
研究的目的:
- 为了调查植物模型植物根球中的植物miRNAs的存在.
- 为了确定这些植物miRNA是否会影响树根球微生物群的组成和功能.
主要方法:
- 在 *Arabidopsis thaliana* 和 *Brachypodium distachyon* 的根球中检测植物miRNA.
- 对细菌吸收合成miRNA和转录基因反应的分析.
- 评估微生物群落在对植物miRNA操纵和体外暴露的反应中发生的变化.
主要成果:
- 植物miRNAs在树根球中被确定并与细菌相关.
- *Variovorax悖论*在暴露于植物miRNA时表现出转录组变化,与*Bacillus mycoides*不同.
- 根球细菌群落的变化在影响小RNA路径或过度表达miRNAs的*Arabidopsis*突变物中被观察到.
结论:
- 植物miRNAs存在于根球中,可以调节居住细菌的基因表达.
- 来自植物的miRNAs有助于塑造根球微生物组合.
- 这项研究突出了由小RNA介导的植物微生物相互作用的新机制.
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