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脂质特征在南暴露斑马鱼胚胎中发生变化,对神经发育和早期生命营养状况有影响
Victoria McGruer1, Anil Bhatia2, Jason T Magnuson3
1Department of Environmental Sciences, University of California, Riverside, California 92521-9800, United States.
Environment & health (Washington, D.C.)
|October 30, 2024
概括
在斑马鱼胚胎中暴露于南会损害黄的利用,减少中性脂质和甘油三. 这表明发育性毒性可能涉及改变脂质代谢和炎症,影响神经元和眼睛发育.
科学领域:
- 环境毒理学环境毒理学
- 发育生物学是发展生物学.
- 利皮多米克 (Lipidomics) 是一种消化剂.
背景情况:
- 乳糖养鱼胚胎的发育取决于母体黄的发育.
- 有毒物质会破坏黄体吸收,影响胚胎发育.
- 芬南是一种心脏毒性化合物,具有潜在的发育影响.
研究的目的:
- 为了研究氨酸如何影响斑马鱼胚胎的黄体利用和脂质代谢.
- 评估改变脂质调动对胚胎发育的影响.
- 探索脂质变化和观察到的发育损伤之间的联系.
主要方法:
- 斑马鱼胚胎在受精后6至72小时内暴露在氨酸中.
- 通过Oil Red O染色来可视化中性脂质.
- 在胚胎体和切割的黄囊上进行了非向性脂质分析.
- 使用生物信息路径分析来解释脂质组数据.
主要成果:
- 暴露于氨酸显著降低了黄囊和胚胎体内的总代谢物和中性脂质.
- 在胚胎体和蛋黄中,甘油三水平都降低了.
- lysophosphatidylcholines 和 lysophosphatidylethanolamines 在 72 hpf 胚胎体内增加.
- 观察到脂质调动减少,在测试剂量下独立于心脏功能.
结论:
- 南会破坏斑马鱼胚胎发育中的脂质代谢和黄体利用.
- lysophospholipids 的增加表明与炎症和脱髓化相关的途径.
- 这些脂质变化可能是之前观察到的神经发育和眼部毒性的基础.
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