菌 (Enterococcus faecium) 和其细胞外囊对乙醇诱导的肠道损伤的保护作用
Meiying Luo1, Suqian Li1, Junhang Sun1
1School of Animal Science and Technology, Foshan University, Foshan, China.
Foodborne pathogens and disease
|October 30, 2024
概括
菌 (E. faecium) 和它的细胞外囊泡 (EVs) 通过减少炎症和增强大鼠的抗氧化活性来保护大鼠免受乙醇诱导的肠损伤.
科学领域:
- 微生物学 微生物学
- 胃肠病学 胃肠病学
- 细胞生物学 细胞生物学
背景情况:
- 乙醇引起的肠损伤是一个重大问题.
- 菌 (E. faecium) 是一种益生菌,具有潜在的治疗应用.
- 细胞外囊泡 (EVs) 正在成为细菌通信和功能中的关键媒介.
研究的目的:
- 调查E. faecium及其细胞外囊 (EV) 对大鼠乙醇诱导的肠损伤的保护作用.
- 阐明E. faecium及其EV的保护作用背后的机制,重点关注肠道形态,抗氧化功能,炎症和透性.
- 确定EVs在调解E. faecium的有益作用中的作用.
主要方法:
- 一个为期5天的食实验,涉及72只雌性Sprague Dawley大鼠,分为9组.
- 使用口服绝对乙醇诱导肠道损伤.
- 用E. faecium,其细胞外囊泡 (EfmEVs) 或酸盐缓冲盐水 (PBS) 进行治疗.
- 评估肠道形态结构,抗氧化酶活性 (超氧化脱酶/马隆迪化),炎症标志物和肠道透性.
主要成果:
- 用E. faecium进行预处理逆转了乙醇诱导的肠道形态变化,并增强了抗氧化能力.
- E. faecium及其EV显著降低了炎症,并增加了肠道的通透性.
- 确定了EVs作为E. faecium对肠道损伤的保护性反应的关键调解者.
结论:
- 在老鼠中,Enterococcus faecium对乙醇诱导的肠损伤提供了显著的保护.
- 由E. faecium分泌的细胞外囊泡在调解这些保护作用方面发挥着至关重要的作用.
- E. faecium及其EVs通过减轻炎症和增强抗氧化剂防御来管理肠道损伤,这是一种有前途的治疗策略.
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