在酵母中生物活性美二烯巴库基的生物合成De Novo
Yi-Lei Zheng1, Ye Xu1, Yan-Qiu Liu1
1First Affiliated Hospital and Institute of Pharmaceutical Biotechnology, Zhejiang University School of Medicine, Hangzhou 310058, China.
ACS synthetic biology
|October 30, 2024
概括
研究人员从葡萄糖中改造了酵母,从葡萄糖中产生化品成分巴库奇醇 (BAK). 这种可持续的微生物生产实现了117.3倍的增长,为传统方法提供了一个环保的替代方案.
科学领域:
- 生物技术是生物技术.
- 自然产品的合成自然产品的合成
- 代谢工程是代谢工程.
背景情况:
- 基醇 (BAK) 是一种美烯,在化学中因其类似于视网醇的效果而受到重视,但由于天然丰度低和生产方法不可持续,面临供应挑战.
- 目前的 bakuchiol 提取和合成方法对环境负担很大,效率低下,需要采用替代生产策略.
研究的目的:
- 开发一个可持续的微生物生产平台,使用工程酵母来生产bakchiol (BAK).
- 为代谢工程识别和描述参与巴库基醇生物合成的关键酶.
主要方法:
- 鉴定了一种来自Psoralea corylifolia的prenyltransferase (PT),它催化了巴库基醇的形成.
- 从葡萄糖中进行巴库基醇生物合成的Saccharomyces cerevisiae.
- 采用了包括提升前体供应,路径流量平衡,酶工程和酶融合在内的战略,以优化生产.
主要成果:
- 在工程酵母菌株中实现了显著的117.3倍 bakuchiol 生产的改善.
- 从葡萄糖开始,达到9.28 mg/L的最终巴库基醇标位.
- 证明了微生物合成复杂甲烯的可行性.
结论:
- 通过人工酵母成功建立了一种可持续和高效的方法,通过人工酵母生产巴库奇醇.
- 这种代谢工程方法为大规模生产有价值的天然产品提供了可行的替代方案.
- 开发的平台可以适应可持续的微生物合成其他复杂的美罗特.
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