相关实验视频

Updated: Jun 9, 2025

Preparation of Exosomes for siRNA Delivery to Cancer Cells

Preparation of Exosomes for siRNA Delivery to Cancer Cells

Published on: December 5, 2018

为瘤免疫疗法提供工程外体:从优化瘤衍生外体到提供CRISPR/Cas9系统

Mingyang Jiang¹, Ke Zhang¹, Jinfeng Meng²

¹Department of Bone and Joint Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.

International journal of cancer

|October 30, 2024

概括

瘤衍生的外体细胞 (TEXs) 是为癌症治疗而设计的,作为CRISPR/Cas9.9等治疗的输送载体. 这些修改后的外基因组在体内克服了有效的基因组编辑和改善癌症治疗结果的挑战.

科学领域:

生物医学工程生物医学工程
癌症生物学癌症生物学
分子治疗学分子治疗学

背景情况:

瘤衍生的外生体 (TEXs) 在癌症进展中起着至关重要的作用,包括瘤生长,免疫逃避,血管生成和药物耐药性.
通过促进特定蛋白质的产生,TEXs促进了癌症的进展.
外体的独特特征允许对其进行治疗应用的修改.

研究的目的:

通过各种工程策略探索增强TEX的技术.
讨论将工程TEX纳入先进的基因工程系统,特别是CRISPR/Cas9.
突出工程外体在癌症治疗中的治疗潜力.

主要方法:

对TEXs的遗传修饰.
使用TEXs开发药物输送系统.
膜,免疫系统和TEXs的化学变化.
在工程外生体中整合CRISPR/Cas9系统.

主要成果:

工程外生体显示承诺作为有效的治疗剂的体内输送载体.
修改策略增强了TEX的针对药物输送和基因工程的能力.
克服像降解和免疫反应这样的体内挑战对于治疗成功至关重要.

结论:

工程TEX为向癌症治疗提供了一个有前途的平台.
外体工程和CRISPR/Cas9的结合为癌症治疗提供了一种新的方法.
对外体工程策略的进一步研究对于实现其全部治疗潜力至关重要.

关键词:

这是一个CRISPR/Cas9系统.工程外生体的工程外生体外基因组是外基因组的组成部分.瘤免疫疗法瘤免疫疗法

更多相关视频

Production of Human CRISPR-Engineered CAR-T Cells

Production of Human CRISPR-Engineered CAR-T Cells

Published on: March 15, 2021

Using CRISPR/Cas9 to Knock Out GM-CSF in CAR-T Cells

Using CRISPR/Cas9 to Knock Out GM-CSF in CAR-T Cells

Published on: July 22, 2019

相关概念视频

Tumor Immunotherapy

Tumor Immunotherapy

Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...