单次使用的聚乙烯四甲酸瓶衍生的纳米塑料通过转化和外膜囊泡分泌在细菌中传播抗生素耐药性
Prashant Sharma1, Abhinoy Kishore1,2, Manish Singh1
1Chemical Biology Unit, Institute of Nano Science and Technology (INST), Knowledge City, Sector-81, Mohali, 140306, Punjab, India. manish@inst.ac.in.
Nanoscale
|October 30, 2024
概括
一次性塑料瓶产生纳米塑料,传播抗生素耐药性. 这些纳米塑料在细菌中促进基因转移,可能会加剧全球抗生素耐药性危机,并影响人类健康.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 毒理学 毒理学 毒理学
背景情况:
- 一次性塑料瓶 (SUPB) 有助于纳米塑料 (NP) 污染.
- 农产品引起了人们对各种环境 (包括人类肠道) 中微生物的毒性和相互作用的担忧.
- 了解NP微生物相互作用对于解决塑料污染对生态和健康的影响至关重要.
研究的目的:
- 研究NP在传播抗生素耐药性 (AR) 中的作用.
- 通过受NP影响的外膜囊泡 (OMV) 探索AR基因传播的机制.
- 分析聚乙烯二甲 (PET) 衍生纳米塑料 (PBNPs) 对细菌基因转移的影响.
主要方法:
- 从PET SUPBs合成PBNPs,模仿环境纳米塑料.
- 通过转化和OMVs进行的PBNP中介水平基因转移 (HGT) 被调查.
- 在暴露于PBNPs的大肠杆菌中分析了与压力反应和OMV分泌相关的基因表达.
主要成果:
- 通过转化和OMV传输,PBNP促进了HGT在细菌之间转化和OMV传输等离子体.
- PBNPs诱导氧化应激和细菌表面损伤,上调应激反应基因和大肠杆菌OMV分泌.
- 通过PNNP,从大肠杆菌转移到Lactobacillus acidophilus的跨物种基因转移.
结论:
- 纳米塑料,特别是PNNP,是传播AR基因的新途径.
- PBNPs有助于全球抗生素耐药性危机,并对人类肠道微生物群构成风险.
- 这些发现突显了纳米塑料在AR中未被认可的作用,并为减轻塑料污染对健康和环境的影响的策略提供了信息.
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