代谢物增强氧化应激,使抗生素耐受性黄金葡萄球菌对诺基诺具有敏感性
Jonathan I Batchelder1, Andrew J Taylor1,2, Wendy W K Mok1
1Department of Molecular Biology and Biophysics, UConn Health, Farmington, Connecticut, USA.
mBio
|October 30, 2024
概括
将营养添加到饥饿的金黄色葡萄球菌 (金黄色葡萄球菌) (S. aureus) 中,通过产生活性氧物种 (ROS),增加了它们对化诺 (FQs) 的敏感性. 这一发现为打击抗生素耐药性细菌感染提供了新的策略.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 抗生素耐药的细菌感染,包括由黄金葡萄球菌 (Staphylococcus aureus) 的持久细胞引起的,由于治疗失败和复发而构成重大威胁.
- 用持久物丰富的静止相细菌培养物对诺基诺 (FQs) 等抗生素的敏感性降低.
- 以前的研究表明,外源营养刺激可以降低细菌对某些抗生素的持久性.
研究的目的:
- 调查营养添加对缺乏营养的金黄色葡萄球菌对化诺 (FQs) 敏感性的作用.
- 阐明S. aureus对FQs,特别是delafloxacin的营养介导敏感化背后的机制.
主要方法:
- 缺乏营养的金黄色葡萄球菌种植被补充了葡萄糖和氨基酸.
- 评估了对诺基诺 (FQs) 的敏感性,包括delafloxacin.
- 分析了核酸合成,膜潜力,活性氧物种 (ROS) 生成以及铁化,基清除和氧气限制的影响.
主要成果:
- 营养补充剂显著提高了S. aureus对FQs的敏感性,尽管增加了核酸合成.
- 在FQ治疗期间,添加营养素增加了膜潜力,并促进了ROS生成.
- 针对ROS的干预措施,如铁化和基清除,从FQ介导的杀戮中拯救了营养刺激的S. aureus.
结论:
- 营养刺激使静止相S. aureus对FQs敏感,主要是通过生成ROS,这可能是由代谢上调驱动的.
- 增加ROS产量,而不是增加核酸合成,是营养物质对FQs传递敏感性的关键因素.
- 针对细菌新陈代谢和增加氧化应激呈现出有希望的策略,以增强FQ有效性对抗抗生素耐受性黄金色杆菌感染.
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