三糖多叶酸参与了Mycobacterium的健康和发病过程
Silke Malmsheimer1, Wassim Daher1,2, Yara Tasrini1
1Centre National de la Recherche Scientifique UMR 9004, Institut de Recherche en Infectiologie de Montpellier (IRIM), Université de Montpellier, Montpellier, France.
mBio
|October 30, 2024
概括
三糖多聚合物 (TPPs) 对于巨细胞中的Mycobacterium的生存和毒性至关重要. 在斑马鱼模型中,TPPs的丧失损害了巨细胞内生存,并降低了感染的严重程度.
科学领域:
- 微生物学 微生物学
- 病变的发生和发病.
- 菌根菌感染 菌根菌感染
背景情况:
- 菌根菌具有多样化的表面脂质,具有重要的生物作用.
- 三醇多聚合物 (TPP) 是表面脂质,参与菌体BPs.的Mycobacterium疹感染.
- 在宿主病原体相互作用和真菌细菌毒性中TPPs的确切作用仍然在很大程度上是未知的.
研究的目的:
- 研究TPP缺失对Mycobacterium abscessus与巨菌相互作用的影响.
- 在体内确定TPPs在Mycobacterium abscessus毒性中的作用.
- 阐明TPPs在宿主感染机制中的功能.
主要方法:
- 在Mycobacterium abscessus中删除mmpL10基因以废除TPP生产.
- 使用人类THP-1细胞和斑马鱼胚胎进行感染检测.
- 分析了内细胞存活率,体酸性化和与加勒-3的同局部化.
- 在斑马鱼模型中评估细菌负担和宿主存活率.
主要成果:
- mmpL10突变体在THP-1细胞中显著降低了巨细胞内生存率和较低的感染率.
- 粗的mmpL10突变显示了受损的体酸性化,并且未能与galactin-3共同局部化.
- 被临床mmpL10突变病毒感染的斑马鱼胚胎显示生存率和细菌负载降低.
- 对于Mycobacterium abscessus的毒性和巨细胞的存活,TPP的产生是必不可少的.
结论:
- 三醇多聚合物 (TPP) 是Mycobacterium abscessus的关键毒性因素.
- TPPs在调节胞体与宿主相互作用方面发挥作用,包括胞体融合和膜损伤.
- 这项研究强调TPP是理解和打击Mycobacterium abscessus病变的重要目标.
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