甲基二醇新代谢物通过体外人体显微体检测,并使用液体染色学/高分辨率质谱学进行特征测定,用于兴奋剂控制目的
Xiya Yan1, Jinghua Hou1, Siyu Yan1
1School of Sport Science, Beijing Sport University, No.48 Xinxi Road, Beijing 100084, China; Beijing Anti-Doping Laboratory, Beijing Sport University, No.1 An Ding Road, Beijing 100029, China.
Journal of pharmaceutical and biomedical analysis
|October 30, 2024
概括
禁止使用的类固醇甲基氨,在人类肝细胞中经历生物转化. 研究人员确定了关键的I阶段和II阶段代谢物,包括18-nor形式,对于兴奋剂控制检测至关重要.
科学领域:
- 药理学 药理学是指药理学的学科.
- 生物化学 生物化学
- 分析化学 分析化学
背景情况:
- 甲基氨是一种强大的合成安卓类固醇 (AAS),被世界反兴奋剂机构 (WADA) 禁止使用.
- 了解其代谢对于有效的兴奋剂控制至关重要.
- 使用人类肝细胞的体外研究为药物生物转化途径提供了洞察力.
研究的目的:
- 用人类肝细胞显微体研究甲基二醇的体外生物转化.
- 为了识别和表征甲基三enolone的I阶段和II阶段代谢产物.
- 探索在兴奋剂分析中检测甲基三enolone的潜在的长期生物标志物.
主要方法:
- 甲基二enolone与人类肝细胞微观体的化.
- 使用液体染色学/高分辨率质谱法 (LC-HRMS) 分析样品.
- 用甲胺或胺衍生代谢物来增强检测灵敏度.
主要成果:
- 确定了七个不同的I阶段代谢物组.
- 代谢途径包括表皮化,化和还原.
- 暂时确定了18-nor代谢物,并提出作为潜在的长期生物标志物.
- 第二阶段的葡萄糖化物代谢物也成功获得和分析.
结论:
- 这项研究阐明了甲基二醇在人类肝脏显微体中的代谢命运.
- 对于抗兴奋剂工作中的扩展检测窗口来说,18-nor甲基二enolone代谢物是有希望的目标.
- 综合性代谢物分析有助于开发用于类固醇检测的强有力的分析方法.
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