相关实验视频

Updated: Jun 9, 2025

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells

Published on: May 1, 2020

eIF3d专用翻译需要一个RACK1驱动的eIF3d结合43S PIC在增殖SH-SY5Y神经母细胞瘤细胞

Federica Silvestri¹, Raffaele Montuoro², Elisabetta Catalani¹

¹Department for Innovation in Biological, Agro-food and Forest systems (DIBAF), Università degli Studi della Tuscia, Viterbo, Italy.

Cellular signalling

|October 30, 2024

概括

当真核启动因子4E (eIF4E) 被抑制时,核糖体RACK1将eIF3d招募到启动前复合体中. 活性蛋白激酶CβII (PKCBII) 促进这种RACK1-eIF3d相互作用,揭示了RACK1在专门翻译中的新作用.

科学领域:

分子生物学分子生物学
细胞生物学细胞生物学
生物化学生物化学

背景情况:

大多数哺乳动物的信使RNA (mRNA) 翻译启动依赖于5'帽结构结合的真核细胞启动因子4E (eIF4E).
尽管eIF4E受抑制,但许多mRNA仍然被封闭,这表明存在其他依赖封闭的转化途径.

研究的目的:

在eIF4E被抑制时,研究依赖上限mRNA翻译的替代机制.
确定核糖体蛋白在翻译调节中的新型作用.

主要方法:

在哺乳动物细胞中使用生物化学分析研究了蛋白质相互作用.
利用eIF4E和PKCBII的抑制来研究翻译启动通路.
专注于RACK1和eIF3d在43S预启动综合体中的作用.

主要成果:

核糖体支架 RACK1在eIF4E抑制后将真核转化启动因子3d (eIF3d) 招募到43S预启动复合体中.
活性蛋白激酶CβII (PKCBII) 对于促进RACK1-eIF3d相互作用至关重要.
发现了核糖体RACK1在促进eIF3d介导的专用翻译方面的新功能.

结论:

这些发现揭示了一条以前未被识别的,对cap-dependent翻译启动的途径.
核糖体RACK1在招募eIF3d方面发挥着关键作用,受活动PKCBII的调节.
这种机制为独立于eIF4F复合体的专门mRNA翻译调节提供了新的见解.

关键词:

在HSP-70中使用.在RACK1中,使用RACK1.翻译控制翻译控制在eIF3d中,您可以使用eIF3d.在 eIF4E 中,您可以使用 eIF4E.在 mTORC1 的情况下,mTORC1 是

更多相关视频

Xenopus laevis as a Model to Identify Translation Impairment

Xenopus laevis as a Model to Identify Translation Impairment

Published on: September 27, 2015

Rapid In Vivo Fixation and Isolation of Translational Complexes from Eukaryotic Cells

Rapid In Vivo Fixation and Isolation of Translational Complexes from Eukaryotic Cells

Published on: December 25, 2021

相关概念视频

Initiation of Translation

Initiation of Translation

Initiating translation is complex because it involves multiple molecules. Initiator tRNA, ribosomal subunits, and eukaryotic initiation factors (eIFs) are all required to assemble on the initiation codon of mRNA. This process consists of several steps that are mediated by different eIFs.
First, the initiator tRNA must be selected from the pool of elongator tRNAs by eukaryotic initiation factor 2 (eIF2). The initiator tRNA (Met-tRNAi) has conserved sequence elements including modified bases at...

Leaky Scanning

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...

Directing Proteins to the Rough Endoplasmic Reticulum

Directing Proteins to the Rough Endoplasmic Reticulum

The organelle-specific signaling sequences direct proteins synthesized in the cytosol to their final destination like ER, mitochondria, peroxisomes, etc. Some of the proteins directed to ER are then trafficked via vesicles to other organelles within the cell or the extracellular environment through the Golgi complex. For example, the rough ER synthesizes soluble proteins for transportation to the lysosomes or secretion out of the cell. It can also synthesize transmembrane proteins that can...

Post-translational Translocation of Proteins to the RER

Post-translational Translocation of Proteins to the RER

A sizable fraction of proteins destined for ER are first synthesized in the cell cytosol and then transported across the ER membrane–a process called post-translational translocation. Similar to cotranslationally translocated proteins, these proteins also use the Sec translocon complex to enter the ER lumen.
Targeting proteins to the ER
Hsp40 and Hsp70 chaperone molecules bind the translated proteins in the cytosol to prevent their folding. The chaperone binding helps to keep the signal...