设计融合双链RNAs来控制两个全球吸害虫
Qin-Qin Xu1, Feng Shang1, Si-Ying Feng1
1Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, China; Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River (Ministry of Education), Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China.
Pesticide biochemistry and physiology
|October 30, 2024
概括
这项研究开发了一种新的RNA干扰 (RNAi) 策略,针对桃花和白中的多个基因. 这种方法增强了害虫控制和作物耐药性,为管理吸水昆虫提供了可持续的解决方案.
科学领域:
- 农业昆虫学 农业昆虫学
- 分子生物学分子生物学
- 虫害管理 虫害管理 虫害管理
背景情况:
- RNA干扰 (RNAi) 是一种用于害虫控制的强有力的基因沉默技术.
- 单基因RNAi策略面临的局限性是由于基因表达的变异性和同时的害虫感染.
- 需要采用多基因,多害虫RNAi方法来提高害虫管理的有效性.
研究的目的:
- 开发一种双害虫RNAi战略,针对桃花 (Myzus persicae) 和白 (Bemisia tabaci) 的同类基因.
- 设计和测试融合双链RNAs (dsRNAs) 以提高沉默效率和减少目标外影响.
- 评估病毒诱导基因沉默 (VIGS) 在提供融合dSRNA用于作物保护方面的有效性.
主要方法:
- 在桃花和白中对同源基因进行全基因组选.
- 用RNAi生物测试来识别诱导高死亡率的有效目标基因 (MpAbd-A,MpH3,MpRpL27a,MpScr).
- 融合dsRNAs的设计和合成,随后进行序列分析,以评估母 (Propylaea japonica) 的目标外风险.
- 在烟草植物中,病毒诱导的基因沉默 (VIGS) 用于表达融合dsRNA并评估对害虫的抵抗力.
主要成果:
- 沉默四个选定的基因 (MpAbd-A,MpH3,MpRpL27a,MpScr) 导致了桃花和白的显著死亡率.
- 与单基因沉默相比,融合dsrna在两种害虫物种中都显示出更高的死亡率.
- 序列分析表明,对掠食性黄虫 (Propylaea japonica) 的目标外风险最小.
- 表达融合dRNA的烟草植物对桃花和白都表现出增强的抵抗力.
结论:
- 一种新的双目标RNAi策略有效地同时控制桃花和白.
- 融合dRNAs为综合性害虫管理提供了比单基因RNAi更有效和更安全的替代方案.
- 融合dsRNAs的VIGS介导表达为开发耐药作物品种提供了一个有希望的途径.
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