在Colletotrichum camelliae分离物中TOR复合1组件的差异表达赋予了对拉帕米辛的自然耐药性
Yuanye Zhu1, Yanjun Li2, Hong Liu1
1College of Forestry, Central South University of Forestry and Technology, Changsha, China; Key Laboratory of National Forestry, Grassland Administration on Control of Artificial Forest Diseases and Pests in South China, Changsha, China; Hunan Provincial Key Laboratory for Control of Forest Diseases and Pests, Changsha, China; Key Laboratory for Non-Wood Forest Cultivation and Conservation of Ministry of Education, Changsha, China.
Pesticide biochemistry and physiology
|October 30, 2024
概括
大多数Collettotrichum真菌表现出对免疫抑制药物拉帕素的天然耐药性. 耐药菌株中较高的TOR同类表达和TORC1信号解释了这种耐药性,为新的杀菌剂开发提供了洞察力.
科学领域:
- 农业科学 农业科学
- 菌类学 菌类学是指菌类学.
- 生物化学 生物化学
背景情况:
- 拉帕米辛是一种宏类抗生素,具有免疫抑制和抗癌性质.
- 最近的研究突出显示了拉帕米辛对抗植物病原菌的潜力.
- 有限的数据存在于Collettotrichum真菌对拉帕米辛的敏感性.
研究的目的:
- 为了评估116种来自茶油树的Collettotrichum分离物对拉帕米辛的敏感性.
- 调查科莱托里 (Colletotrichum camelliae.) 中拉帕米辛耐药性的基础分子机制.
主要方法:
- 对116种Collettotrichum分离物对拉帕米辛 (50μg/mL) 的敏感性测试.
- 在耐药和敏感分离物中对FKBP12和TOR-FRB域进行基因分析.
- 对CcTOR,核糖体生物发生基因和TORC1组件的基因表达分析.
- 在拉帕治疗下对核糖体蛋白S6酸化的评估.
主要成果:
- 大多数隔离物表现出对拉帕素 (50-70%的抑制) 的天然耐药性.
- 鉴定出了三种敏感的Collettotrichum camelliae分离物.
- 在FKBP12或TOR-FRB域中没有发现突变.
- 耐药分离物表现出更高的CcTOR表达和TORC1丰度,与拉帕米辛敏感性降低相关.
- 拉帕米辛治疗在敏感的分离物中取消了S6酸化,但在耐药的分离物中没有.
结论:
- 科莱托里 (Colletotrichum camelliae) 呈现出对拉帕素的自然耐药性,主要通过增加的CcTOR表达和TORC1信号传递进行介导.
- 了解这些耐药机制可以为农业应用开发新型TORC1信号向杀菌剂提供信息.
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