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巨型淡水 (Macrobrachium rosenbergii) 的染色体水平基因组组合

Shiyan Liu^1,2, Meihui Li¹, Chong Han³

¹State Key Laboratory of Biocontrol and School of Life Sciences, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, Sun Yat-Sen University, Guangzhou, 510275, China.

Scientific data

|October 31, 2024

概括

研究人员已经对巨型淡水 (Macrobrachium rosenbergii) 的染色体级基因组进行了测序. 这种高品质的基因组资源,包括它的性别染色体,将促进分子育种和功能基因组研究,为这个有价值的水产养殖物种.

科学领域:

水产养殖基因组学水产养殖基因组学
甲动物遗传学甲动物遗传学
进行比较基因组学.

背景情况:

巨型淡水 (Macrobrachium rosenbergii) 是一个非常有价值的水产养殖物种,因为它的快速生长,短繁殖周期和营养益处.
高质量的基因组资源对于推进选择性育种计划和了解水产养殖中理想特征的遗传基础至关重要.

研究的目的:

为男性和女性巨型淡水生成染色体水平的基因组组合.
为了识别和描述Macrobrachium rosenbergii.的性别染色体 (Z和W).
为未来的分子育种和功能研究提供基础的基因组学资源.

主要方法:

使用了Illumina配对末端测序,PacBio单分子测序和高通量染色体形状捕获 (Hi-C) 技术的组合.
组装了男性 (ZZ) 和女性 (WW) 的高质量基因组序列.
定了59个染色体的组合序列,并表征了Z和W性别染色体.

主要成果:

获得了男性的3118.58 Mb (N50: 956,237 bp) 和女性的3333.31 Mb (N50: 1,143,555 bp) 的最终结合组.
成功组装和识别了Z染色体 (27.33Mb) 和W染色体 (36.23Mb),它们之间的序列相似性为74.90%.
该基因组被定在59个染色体上,提供了一个全面的染色体图.

结论:

生成的染色体级基因组组合代表了Macrobrachium rosenbergii研究的重大进展.
这种高质量的基因组资源将促进标记器辅助的选择,基因发现和水产养殖改进菌株的开发.
通过这些新的基因组数据,将加速了解性别决定和其他特征的遗传结构.

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