Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Ribosome Profiling02:24

Ribosome Profiling

3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Error characterization and error correction approaches in combinatorial DNA-based storage.

Scientific reports·2026
Same author

High-throughput transcriptome analysis reveals a developmental increase in Acvr1c which mediates epigenetic repression of the gene encoding the pubertal brake, Makorin ring finger protein 3.

NAR molecular medicine·2025
Same author

Latent representation of H&E images retains biological information in a breast cancer cohort.

PloS one·2025
Same author

Pooled CRISPR screens identifies key regulators of bovine stem cell expansion for cultured meat.

Communications biology·2025
Same author

RNA-interactome capture identifies SRSF3 as a key protein for herpesviral gene expression.

PNAS nexus·2025
Same author

Cross-Activity Analysis of CRISPR/Cas9 Editing in Gene Families of <i>Solanum lycopersicum</i> Detected by Long-Read Sequencing.

Current issues in molecular biology·2025
Same journal

Therapeutic potential of crude protein extracts from two Egyptian freshwater snails Lanistes carinatus and Bellamya unicolor.

Scientific reports·2026
Same journal

Microbial contamination of donor corneas and post-keratoplasty endophthalmitis: a comparison between Japanese and U.S. eye banks using cold storage.

Scientific reports·2026
Same journal

Prevalence and contributing factors of virological non-suppression among adult patients on first-line antiretroviral therapy in tertiary hospitals in Ethiopia.

Scientific reports·2026
Same journal

An in vitro comparison of color stability between alkasite and different restorative materials in various staining solutions.

Scientific reports·2026
Same journal

Toward accessible mRNA LNP formulation: systematic evaluation of mixing strategies and key parameters.

Scientific reports·2026
Same journal

A network analysis of personality traits, mentalizing, and psychological health in Chinese college students.

Scientific reports·2026
查看所有相关文章

相关实验视频

Updated: Jun 9, 2025

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

6.6K

通过灵活的计算方法检测单细胞和空间转录学中的显著表达模式.

Hadas Biran1, Tamar Hashimshony2, Tamar Lahav2

  • 1Computer Science Department, Technion - Israel Institute of Technology, Haifa, Israel. hadas.moriah@gmail.com.

Scientific reports
|October 31, 2024
PubMed
概括
此摘要是机器生成的。

SPIRAL是一种新的算法,可以从单细胞,批量和空间转录组的基因表达数据中找到重要的生物过程. 它识别了基因细胞结构,揭示了其他方法错过的微妙但重要的生物学见解.

更多相关视频

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

18.5K
An Approach to Study Shape-Dependent Transcriptomics at a Single Cell Level
06:02

An Approach to Study Shape-Dependent Transcriptomics at a Single Cell Level

Published on: November 2, 2020

5.7K

相关实验视频

Last Updated: Jun 9, 2025

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

6.6K
Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

18.5K
An Approach to Study Shape-Dependent Transcriptomics at a Single Cell Level
06:02

An Approach to Study Shape-Dependent Transcriptomics at a Single Cell Level

Published on: November 2, 2020

5.7K

科学领域:

  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.
  • 计算生物学 计算生物学

背景情况:

  • 基因表达数据提供了对生物过程的洞察力.
  • 目前的单细胞和空间转录组学分析方法主要集中在聚类或识别主要数据趋势上.
  • 这些方法可能忽略了涉及细胞或样本子集的重要生物过程.

研究的目的:

  • 引入SPIRAL (显著过程感染算法),一种用于在各种转录组学数据类型中检测统计学上显著的生物过程的新方法.
  • 提供一种灵活的算法,能够识别复杂的基因细胞结构.

主要方法:

  • 螺旋利用高斯统计来检测重要的生物过程.
  • 它识别了特定细胞群中由共同表达的基因定义的结构.
  • 算法通过在基因和细胞子集中选择统计学上显著和一致的差异表达来构建这些结构.

主要成果:

  • SPIRAL成功地检测到单细胞,批量和空间转录学数据中的所有统计学上显著的生物过程.
  • 算法输出定义的结构,每个结构包含一组在特定细胞群中运行的基因.
  • 在合成和现实数据集上的验证证实了SPIRAL的统计学稳定性和实用性.

结论:

  • 螺旋提供了一种灵活而强大的方法,用于从转录学数据中发现隐藏的生物过程.
  • 它识别基因细胞结构的能力增强了对复杂生物系统的理解.
  • 该算法提供了有价值的可视化和路径丰富信息,有助于生物发现.