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冷-X射线相对照成像使得3D结构定量和心肌活检的核酸分析能够结合起来

Kan Yan Chloe Li¹, Petros Syrris¹, Anne Bonnin²

¹Institute of Cardiovascular Science, University College London, London, WC1N 1DZ, UK.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

|October 31, 2024

概括

快速冷活检可以在核酸提取之前使用冷X射线相对照成像 (冷X-PCI) 在3D中进行分析. 这种方法保留了组织结构和遗传物质,有助于疾病研究.

科学领域:

生物医学成像技术生物医学成像技术
分子生物学分子生物学
病理学病理学病理学

背景情况:

快速冷活检对疾病研究至关重要,使核酸分析成为可能.
从活检中获取3D结构信息是具有挑战性的,以获得预先的多体瘤.
传统的组织学对心脏等软组织有局限性,原因是破坏性处理.

研究的目的:

评估基于同步仪的冷X射线相对照成像 (冷X-PCI) 对于快速冷心肌活检的可行性.
评估冷-X-PCI后3D组织结构和核酸的保存情况.
为了确定冷-X-PCI的临床价值,用于活检的核酸前分析.

主要方法:

快速冷的心肌活检使用基于同步仪的冷X-PCI进行准备和分析.
在聚合的肌细胞上进行了3D结构张量分析.
核酸 (DNA和RNA) 被提取并分析成像后.

主要成果:

最佳的样本制备对于保存组织结构和核酸至关重要.
化X-PCI并没有对核酸保存产生负面影响.
心肌组织的3D结构完整性得到维持.

结论:

冷-X-PCI是一种可行的技术,用于快速冷心肌活检的3D分析.
这种方法允许在分子分析之前进行结构性表征,而不会损害核酸.
化X-PCI对软组织活检具有潜在的临床价值,包括心脏和非心脏样本.

关键词:

活体检查活体检查化-X射线相位对比成像成像技术基因组学就是基因组学.核酸分析核酸分析翻译学翻译学翻译学翻译学

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