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全球出现了带有PBP3插入的大肠杆菌

Haiyan Long¹, Feifei Zhao¹, Yu Feng²

¹Center of Infectious Diseases, West China Hospital, Sichuan University, Guoxuexiang 37, Chengdu 610041, China.

The Journal of antimicrobial chemotherapy

|October 31, 2024

概括

在大肠杆菌中插入的青素结合蛋白3 (PBP3) 正在全球传播,通常与NDM-金属β-乳糖酶和高风险克隆有关. 这凸显了迫切需要一个

科学领域:

微生物学微生物学
基因组学就是基因组学.
抗微生物耐药性抗微生物耐药性

背景情况:

产生金属β-乳糖酶 (MBLs) 的大肠杆菌菌株与素结合蛋白3 (PBP3) 插入,显示出对关键抗生素,如阿兹特里-avibactam和cefiderocol.col降低了敏感性.
了解PBP3插入的患病率和遗传特征对于跟踪和打击抗菌素耐药性至关重要.

研究的目的:

分析高质量的大肠杆菌基因组,以识别和描述PBP3插入.
确定在大肠杆菌中PBP3插入的频率,变异和全球分布.

主要方法:

从EnteroBase (n=167,518) 获取基因组,使用CheckM2进行质量控制,并通过fastANI.com确认物种.
测序类型 (ST) 确定使用多位点测序类型和耐药性基因识别使用AMRFinderPlus.
PBP3插入分析涉及编码序列预测 (Prokka),蛋白质序列比较 (BLAST+) 和变异注释 (SnpEff).

主要成果:

在159,341个高质量的大肠杆菌基因组中的2.01% (n=3198) 中发现了PBP3插入,其中有11个不同的变异.
主要变体是334-337氨基酸重复 (94.75%),包括YRIN (65.92%) 和YRIK (28.83%).
在全球范围内发现了插入变体,与85种ST相关,特别是高风险克隆ST410 (29.18%),ST167 (23.40%),ST405 (10.56%),其中83.32%编码NDM金属β-乳糖酶.

结论:

带有PBP3插入的大肠杆菌的全球传播,经常与NDM,高风险ST和多种宿主有关,意味着抗菌素耐药性危机日益严重.
这些发现强调,迫切需要制定全面的"一健康"战略,以应对抗菌素耐药性不断升级的威胁.
持续的基因组监测对于监测这些耐药菌株的传播和为公共卫生干预提供信息至关重要.

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