第一个Bacillus tropicus菌体的重新分类要求重新分类以前被指定为等离子体的其他温带菌体
Ridwaan Nazeer Milase1, Johnson Lin2, Nontobeko E Mvubu3
1Discipline of Microbiology, School of Life Sciences, College of Agriculture, Engineering and Science, University of KwaZulu-Natal (Westville Campus), Private Bag X54001, Durban, 4000, Republic of South Africa. r.n.milase@gmail.com.
BMC genomics
|October 31, 2024
概括
我们提出了三种新的Bacillus菌体物种,包括RBClin15,形成了一个新的属,Theosmithvirus. 这些温带菌体在GenBank数据库中被错误地识别为等离子体.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 病毒学 病毒学
背景情况:
- 热带杆菌 (Bacillus tropicus) 是谷类杆菌的一个亚种,与抗菌素耐药性和类似炭杆菌的疾病有关.
- 此外,B. tropicus 在生物修复和生物转化方面也有潜力.
- 感染B. tropicus的温带菌对于理解细菌进化和菌体与宿主相互作用至关重要.
研究的目的:
- 为了对感染B. tropicus. 的Bacillus菌体vB_Btc-RBClinn15 (RBClin15) 的基因组进行比较.
- 为了识别和分类RBClin15和相关菌体错误地作为GenBank.Bank中的等离子体存储.
- 为这些相关的温带菌体提出一个新属.
主要方法:
- 全基因组测序RBClin15. 的整体基因组测序
- BLASTn对基因银行数据库进行了搜索.
- 基因组比较分析,包括平均核酸标识 (ANI) 和蛋白质同质学.
- 遗传学和基于蛋白质的遗传学树结构.
主要成果:
- 确定RBClin15是一种温带菌体,具有分区和任意系统.
- 在GenBank.中发现其他七种温带菌体被错误地识别为质粒.
- RBClin15与这七种菌体共享87-92%的ANI,形成一个独特的基因组.
- 这些菌体与其他已知的菌体具有较低的蛋白质同质性 (<29%).
结论:
- RBClin15和七个相关的菌体代表了温带菌体的独特血统.
- 遗传学和基因组分析支持了三种新物种的提议.
- 这些物种被提议在Caudoviricetes中形成新的Theosmithvirus属.
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