牛群中的β-Defensin基因拷贝数变化
Ozge Sidekli1, John Oketch1, Sean Fair2
1Department of Genetics and Genome Biology, University of Leicester, Leicester, UK.
Royal Society open science
|October 31, 2024
概括
牛β-防御素 (β-防御素) 基因拷贝数的变化很大,特别是对于DEFB103,影响生育能力. 性成熟期间的基因表达变化表明在生殖特征中发挥了作用.
科学领域:
- 基因组学和蛋白质组学
- 生殖生物学 生殖生物学
- 免疫学 免疫学 免疫学
背景情况:
- β-防御素 (β-defensins) 是抗菌,在免疫和繁殖中起着至关重要的作用.
- 在β-defensin基因中的基因复制数变异 (CNV) 在哺乳动物中很普遍,可能会影响诸如生育能力和抗病能力等特征.
- 之前的研究表明β-defensins参与精子成熟和功能.
研究的目的:
- 综合目录牛β-defensin基因,并分析它们的复制数量在不同品种的变化.
- 调查性成熟期间β-defensin基因的表达模式及其与基因拷贝数的相关性.
- 探索β-防御素CNV对牛的生育能力的功能影响.
主要方法:
- 在牛中目录了55个β-defensin基因,并构建了一个家族遗传树来识别人类的ortologues.
- 在不同牛品种中评估β-防御素基因CNV,重点是DEFB103.
- 对比小牛和成年公牛的丸中的β-defensin基因表达,以及成年公牛的上,将表达与基因拷贝数相关联.
主要成果:
- 鉴定了35种牛β-defensins与1:1的人类正方体,并发现了跨品种的广泛的CNV,其中DEFB103表现出显著的多元基CNV.
- 发现14种β-defensins,包括DEFB103,在性成熟期间表达增加.
- 在成年公牛中,证明了四种β-defensins (包括DEFB103) 的表达水平和基因组拷贝数之间的相关性.
结论:
- 在牛β-防御素基因中存在大量的拷贝数变异,特别是DEFB103.
- 观察到的CNV和表达变化表明β-defensins,特别是DEFB103,在牛的生殖特征和生育能力中起着重要作用.
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