使用含有酸盐的无形酸和凝甲基酸盐开发纳米复合物水凝
Abhishek Indurkar1,2, Kristaps Rubenis1,2, Aldo R Boccaccini3
1Institute of Biomaterials and Bioengineering, Faculty of Natural Sciences and Technology, Riga Technical University, Riga, Latvia.
Frontiers in bioengineering and biotechnology
|October 31, 2024
概括
凝甲基酸盐 (GELMA) 水凝与无形酸酸 (ACP_CIT) 的化学交联为骨组织工程提供了基于UV的方法的可行替代方案. 这种方法可以增强机械性能,而不损害细胞活力.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 纳米技术 纳米技术
背景情况:
- 纳米复合材料水凝模仿细胞外基质,支持骨组织工程的细胞生长.
- 凝甲酸盐 (GELMA) 是一种生物相容的材料,但由于填充剂诱导的不透明性,在更厚的结构中阻碍了光学交叉连接.
- 开发强大的和细胞相容的基凝用于骨再生需要克服交叉连接的局限性.
研究的目的:
- 开发一种化学交联的纳米复合物GELMA水凝,其中包含无形酸酸 (ACP_CIT).
- 评估化学交联的水凝的机械性能和细胞相容性.
- 将化学交叉连接作为光学交叉连接的优越替代品,用于制造更厚的基于GELMA的骨组织工程支架.
主要方法:
- 使用氨硫酸 (APS) 和四甲基乙二胺 (TEMED) 的化学交联的GELMA水凝.
- 将含有酸盐的无形酸盐 (ACP_CIT) 纳入GELMA矩阵中.
- 通过振荡式剪切测试 (存储G'和损失G"模块) 评估机械性能.
- 使用体外分析评估细胞相容性.
主要成果:
- 化学交叉连接显著提高了GELMA水凝的储存和损失模块.
- 加入ACP_CIT进一步提高了水凝的机械强度 (G'和G′′).
- 在体外研究证实,化学交叉连接和ACP_CIT添加都保持了GELMA的细胞相容性.
结论:
- 使用APS/TEMED进行化学交叉连接是一种有效的方法,用于制造骨组织工程的强大的GELMA水凝.
- 加入ACP_CIT可增强机械性能,而不会对细胞活力产生负面影响.
- 这种化学交叉连接的纳米复合材料水凝系统为制造复杂的骨架提供了相片交叉连接的有希望的替代方案.
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