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PbRVE6通过调节关键生物合成基因,促进梨果皮中的安托西亚宁积累

Xieyu Li^1,2, Wei Du^1,2, Yinsheng Cheng^1,2

¹Fruit and Tea Research Institute, Hubei Academy of Agricultural Sciences, Wuhan, Hubei, China.

Physiologia plantarum

|October 31, 2024

概括

REVEILLE (RVE) 转录因子 PbRVE6 通过增加酸积累,促进梨的红皮颜色. 它直接调节关键基因,为开发具有增强红色色彩的新梨品种提供了潜力.

科学领域:

植物分子生物学植物分子生物学
果实遗传学果实遗传学
生物化学生化学

背景情况:

梨的红色皮肤颜色是商业上有价值的特征,由素积累驱动.
REVEILLE (RVE) 转录因子是已知的植物中安托氨酸生物合成的调节者,但它们在梨皮中的特定作用尚未得到充分研究.

研究的目的:

为了研究RVE转录因子在调节梨果皮中的安东氨酸生物合成中的功能.
为了识别特定的RVE基因参与发育红色皮肤颜色在梨突变.

主要方法:

在"Zaosu"梨及其红色突变"红色Zaosu"之间进行基因表达比较分析.
PbRVE6的亚细胞局部化,在梨中过度表达基因,以及VIGS介导的基因沉默.
酵母单杂交和双露西法酶记者测定以确认转录因子促进体相互作用.

主要成果:

与"Zaosu" (低) 梨相比",红色Zaosu" (高) 的PbRVE6表达显著更高.
过度表达PbRVE6增加了安东含量,并调高了PbANS和PbUFGT的表达,而沉默则降低了它们.
证实PbRVE6可以结合并激活PbANS和PbUFGT的促进子,这是安东素通路中的关键基因.

结论:

通过调节PbANS和PbUFGT的表达,PbRVE6直接促进梨皮中安托西亚尼的积累.
这项研究阐明了梨子红皮颜色的关键调节机制.
PbRVE6作为一种潜在的遗传资源,用于培育具有理想红色色彩的新梨品种.

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