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有效的7-脱水胆固醇生产通过多重代谢工程的双胞胎Saccharomyces cerevisiae

Ziqi Ye1,2, Xianhao Xu1,2, Yaokang Wu1,2

  • 1Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, Jiangsu 214122, China.

Journal of agricultural and food chemistry
|October 31, 2024
PubMed
概括

研究人员对酵母进行了改造,以产生7-脱胆固醇 (7-DHC),这是维生素D3的前体. 这种优化的微生物发酵过程实现了创纪录的3.80g/L标位,使7-DHC具有成本效益的大规模制造成为可能.

关键词:
7-脱胆固醇的使用方法二倍体的 糖菌 (Saccharomyces cerevisiae) 是一种埃尔戈斯醇 (Ergosterol) 是一种发酵优化优化发酵的优化代谢工程是代谢工程.

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Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
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