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用Deinococcus deserti的氨基糖酶来有效地生物合成一种强大的益生菌Theanderose,这是一种强大的益生菌

Jeon-Uk Kang¹, Yun-Sang So¹, Gyungcheon Kim¹

¹Department of Food Science and Biotechnology, and Carbohydrate Bioproduct Research Center, Sejong University, 209 Neungdong-ro, Gwangjin-gu, Seoul 05006, Republic of Korea.

Journal of agricultural and food chemistry

|October 31, 2024

概括

研究人员开发了一种高效的生物工艺,使用Deinococcus deserti amylosucrase (DdAS) 合成仙. 这种新型的益生菌选择性地促进了有益的肠道细菌,表明了肠道健康应用的巨大潜力.

科学领域:

生物技术和微生物化学
营养科学和肠道微生物组研究

背景情况:

对于具有特定功能的新型益生菌的需求正在增长.
氨基糖酶是能够合成具有潜在益生菌性质的寡糖酶的酶.
安德罗斯的特定特性和益生菌潜力以前没有被描述.

研究的目的:

开发一种高效的生物工艺,用于发现和合成使用Deinococcus deserti氨基糖酶 (DdAS).
为了表征合成的安德罗斯,并评估其体外消化能力.
评估安德罗斯对有益肠道细菌生长的益生菌疗效及其对人类便微生物群的影响.

主要方法:

使用DDAS和砂糖的安德酶合成.
使用染色学 (HPLC,MPLC) 和光谱学 (NMR,MS) 净化和结构性鉴定.
在体外消化测试,使用Bifidobacterium菌株进行 prebiotic 疗效测试和人类便发酵研究.

主要成果:

在优化条件下 (2.0M糖,40°C,96小时) 一个高效的生物工艺产生了anderose,产量为25.4% (174.1g/L).
安德在体外水解率低 (在16小时内6.5%),并显著促进了Bifidobacterium菌株的扩散.
便发酵表明,安德罗斯可以选择性地增加有益细菌 (Bifidobacteriaceae,Prevotellaceae) 和减少潜在的有害细菌 (Lachnospiraceae,Ruminococcaceae).

结论:

开发的生物工艺提供了一种高效的方法来生产安德罗斯.
的消化能力低,具有选择性益生菌活性,促进有益的肠道微生物群.
仙草显示出作为一种功能性益生菌成分的显著潜力,可以改善肠道健康.

关键词:

迪诺科克沙漠菌 (Deinococcus deserti) 是一种植物.氨基糖酶氨基糖酶是什么便发酵便发酵是什么益生菌益生菌前生物他们的anderose.

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