相关实验视频

Updated: Jun 9, 2025

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

Published on: May 12, 2023

通过四基离子识别G-四体:平行和反平行G-四体之间的歧视的新范式

Xuan Li¹, David N Dubins¹, Jens Völker²

¹Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto, 144 College Street, Toronto, Ontario M5S 3M2, Canada.

The journal of physical chemistry. B

|October 31, 2024

概括

四基离子选择性地结合并稳定了平行c-MYC G-四重复的DNA结构. 这一发现为开发新型拓选择性G-四重复结合剂提供了基础.

科学领域:

分子生物学分子生物学
生物化学生物化学
遗传学遗传学是一个

背景情况:

G四重复是关键的非正规DNA结构,调节基因组事件.
G-四重复和双重复DNA状态之间的相互转换是它们调节功能的关键.
开发用于选择性向G-四重复合体的药物是一个活跃的研究领域.

研究的目的:

为了研究四基 (TAA+) 离子作为G-四体识别剂的潜力.
为了确定TAA+离子是否可以在双重DNA上选择性地稳定特定的G-四重复结构.
探索TAA+离子结构与结合亲和之间的相关性.

主要方法:

循环二极化 (CD) 光谱法被用来研究DNA结构变化和离子结合.
实验使用并行c-MYC G-四重复,反并行人类端粒G-四重复和双重复的DNA进行.
在TAA+离子中测试了不同长度的异质侧链.

主要成果:

四基离子强烈稳定了平行c-MYC G-四重复.
TAA+离子没有与人类反平行端粒G-四重复或双重复的DNA结合.
结合亲和力与TAA+离子的亚利法侧链的长度相关.
CD的光谱数据显示了外部结合,但没有改变G-四重复结构.

结论:

四基离子对G-四重复DNA具有显著的拓选择性.
这些离子可以区分不同的G-四重复结构和双重复DNA.
TAA+盐作为开发拓选择性G-四重复结合剂的有希望的起点.

更多相关视频

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1

Published on: March 18, 2017

相关概念视频

Mass Analyzers: Common Types

Mass Analyzers: Common Types

The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...

Structure of Amines

Structure of Amines

The hybridized nitrogen atom in amines possesses a lone pair of electrons and is bound to three substituents with a bond angle of around 108°, which is less than the tetrahedral angle of 109.5°. However, the C–N–H bond angle is slightly larger at 112°, with a carbon–nitrogen bond length of 147 pm. This carbon–nitrogen bond length of of amines is longer than the carbon–oxygen bond of alcohols (143 pm) but shorter than alkanes’...

Maxam-Gilbert Sequencing

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...