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通过Phanerochaete chrysosporium与天然 siderite解读 thiamethoxam 的生物降解:协同作用机制,转录学特性和分子模拟

Shiye Zhu¹, Anwei Chen¹, Jiale Zhang¹

¹College of Environment and Ecology, Hunan Agricultural University, Changsha 410128, PR China.

Journal of hazardous materials

|October 31, 2024

概括

白色腐烂真菌Phanerochaete chrysosporium和 siderite协同增强了 thiamethoxam (THX) 农药的去除. 赛德里特促进了真菌酶和基因表达,改善了弹性和污染物降解.

科学领域:

环境微生物学环境微生物学
生物地质化学生物地质化学
生物修复是一种生物修复.

背景情况:

在土壤和水生环境中,真菌对有机污染物的降解至关重要.
在污染物减轻过程中,真菌与矿物相互作用的机制尚不清楚.

研究的目的:

研究Phanerochaete chrysosporium和 siderite对 thiamethoxam (THX) 转化的协同作用.
阐明真菌-矿物相互作用在增强污染物生物降解和真菌弹性方面的作用.

主要方法:

使用P. chrysosporium和 siderite的共同培养系统来研究THX降解.
使用了酶活性测定,基因表达分析和分子动力学模拟.
监测了THX去除速率和降解途径.

主要成果:

与 siderite 共同培养显著增强了 THX 清除,在 25 天内达到 86%.
赛德里特增加了真菌的解酶和细胞染色体P450活性.
与真菌生长,新陈代谢和应激反应相关的基因表达被上调,增强了THX弹性.

结论:

赛德里特作为一种天然增强剂,增强真菌对污染物降解和抗压能力的能力.
菌类与矿物质的相互作用为环境污染物减弱和理解生物矿物化过程提供了有希望的策略.

关键词:

生物接触可以达到生物接触.菌类的适应性菌类的适应性菌类与矿物质的相互作用模拟MD的模拟方法尼可类杀虫剂是一种新型农药.

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