酶诱导碳酸盐沉 (EICP) 与不同尿酶源对修复的影响
Yanbo Chen1, Qingyang Wang2, Yi Bian2
1MOE Key Laboratory of Soft Soils and Geoenvironmental Engineering, Zhejiang University, Hangzhou, China; Center for Hypergravity Experiment and Interdisciplinary Research, Zhejiang University, Hangzhou, China.
Journal of hazardous materials
|October 31, 2024
概括
剑豆尿酶 (SU) 通过酶诱导碳酸盐沉 (EICP) 有效地固定离子 (Zn2+). 在 Zn2+ 修复中,SU 优于 jack bean 尿素酶 (JU),通过生物矿化和吸附实现了超过 98% 的固定.
科学领域:
- 环境科学 环境科学
- 生物技术是生物技术.
- 地质化学 地质化学
背景情况:
- 酶诱导碳酸盐沉 (EICP) 是重金属修复的一个有前途的技术.
- 尿素酶在EICP中发挥着至关重要的作用,通过催化尿素的水解,导致碳酸盐沉.
- 调查不同的尿酶来源对于优化EICP效率至关重要.
研究的目的:
- 评估使用EICP的离子 (Zn2+) 补救中杰克豆尿酶 (JU) 和剑豆尿酶 (SU) 的疗效.
- 为了比较SU和JU在不同条件下固定Zn2+的性能.
- 阐明涉及到Zn2+被SU固定的机制.
主要方法:
- 酶诱导碳酸盐沉 (EICP) 使用JU和SU.
- 用不同度的尿酶和化处理Zn2+溶液.
- 对 (NH4+) 转换效率和 Zn2+ 固定百分比的分析.
- 使用X射线衍射 (XRD),富里埃变换红外光谱 (FTIR) 和扫描电子显微镜与能量分散光谱 (SEM-EDS) 进行沉产品的表征.
主要成果:
- 剑豆尿酶 (SU) 显示显著更高的Zn2+固定性 (95.1%高于JU) 和NH+转化效率.
- 在SU中存在有机分子保护了尿酶,并有助于Zn2+的吸收.
- 与化一起,SU促进了99%以上的碳酸 (CaCO) 形成,导致98.9%的Zn2+固定.
- XRD,FTIR和SEM-EDS证实了CaCO,碳酸盐 (ZnCO) 和氧化 (ZnOH) 的形成.
结论:
- 剑豆尿素酶 (SU) 与豆尿素酶 (JU) 相比,是Zn2+固定化的优越催化剂.
- 在SU中的高有机含量增强其保护性和吸收性,用于 Zn2+ 补救.
- Zn2+ 固定发生通过生物矿化 (形成ZnCO3和Zn(OH) 2) 和吸附到CaCO3和SU有机分子.
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