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关于生中介性 ochratoxin A 的动力学研究和优化:包括毒性评估在内的环保方法

Ashutosh Bahuguna¹, Vishal Kumar¹, Sumi Lee¹

¹Department of Food Science and Technology, Yeungnam University, Gyeongsan, Gyeongsangbuk-do, 38541, Republic of Korea.

|October 31, 2024

概括

生汁有效地降低了毒性食品污染物Ochratoxin A (OTA). 这种天然化合物减轻了OTA的作用.

科学领域:

食品安全和毒理学
自然产品化学自然产品化学
菌毒素的研究研究.

背景情况:

毒素A (OTA) 是一种在食品和料中发现的2B组致癌性菌毒素.
OTA对人类和动物构成重大健康风险.
减少OTA污染的有效方法对于公共卫生至关重要.

研究的目的:

选食用植物材料,以检测奥克拉托克素A的降解活性.
调查生汁减少OTA的动力学和影响因素.
评估生在减轻OTA毒性的疗效,在体外和体内.

主要方法:

对7种食用植物材料进行选,以减少OTA.
使用第一阶动力学的生汁减少OTA的动力学分析.
使用响应表面方法 (RSM) 和盒子-Behnken设计,优化OTA减少条件.
评估生汁成分的热稳定性和分子量.
对治疗OTA对HepG2细胞和斑马鱼胚胎的影响的评估.

主要成果:

生汁在第一阶级动力学之后,显示出最大的OTA降低 (95.63%) 达到2.5% (v/v).
盐 (>2.5%) 和温度 (>40°C) 对OTA的减少产生了负面影响.
RSM分析显示了OTA减少的最佳条件,观察到的减少范围从27.44%到100%.
生汁中的热稳定小分子有助于减少OTA.
用生治疗的OTA改善了肝细胞癌细胞活力,减少了反应性氧物种 (ROS),并在斑马鱼胚胎中逆转了OTA诱导的病原性和死亡率.

结论:

生汁是一种强大的天然剂,可以减少食品中的Ochratoxin A污染.
生汁的功效受盐度和温度的影响.
生治疗显著减少了Ochratoxin A的毒性,为食品安全提供了一个有希望的方法.
对负责OTA降低和排毒的特定化合物的进一步研究是有必要的.

关键词:

细胞毒性细胞毒性生是什么意思生是什么意思生是什么意思生是什么意思奥克拉托克辛A是一种减少减少减少原性效应原性影响斑马鱼是一种斑马鱼.

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