对于活体中的核糖体生物发生而言,需要Drosophila核糖核蛋白Clueless
Aditya Sen1, Ambar Rodriguez-Martinez1, Sara K Young-Baird2
1Department of Biochemistry and Molecular Biology, Uniformed Services University, Bethesda, Maryland, USA; Henry M. Jackson Foundation, Rockville, Bethesda, USA.
The Journal of biological chemistry
|October 31, 2024
概括
果无痕 (Clu) 对于核糖体生物发生和全球蛋白质合成至关重要. 这项研究揭示了Clu.
科学领域:
- 线粒体生物学 线粒体生物学
- 分子和细胞生物学分子和细胞生物学
- 核糖体生物生成 (Ribosome Biogenesis) 是一个过程.
背景情况:
- 线粒体是细胞代谢和能量生产的核心.
- 草无线虫 (Clu) 和脊椎动物的CLUH是支持线粒体功能的核核蛋白蛋白.
- 克卢-克卢H结合mRNAs,而克卢H调节了线粒体蛋白的mRNA局部化和翻译.
研究的目的:
- 为了研究Clu相互作用体及其在核糖体生物发生中的作用.
- 为了确定Clu是否需要在体内进行基本的核糖体功能.
- 阐明Clu在细胞功能中的多方面的作用.
主要方法:
- 分析了Clu相互作用组的分析.
- 研究clu null突变体以评估蛋白质合成.
- 研究Clu与核糖体蛋白和rRNA处理因子的关联.
- 核糖体子单元丰度的量化.核糖体子单元丰度的量化.
主要成果:
- 克鲁以独立于mRNA的方式与核糖体蛋白质结合.
- 克鲁零突变体的全球蛋白质合成显著下降.
- 克鲁存在于细胞核中,并与纤维激素相互作用.
- 结核损失降低了40S和60S核糖体亚单元的丰富性,但不会影响加工的rRNA或核糖体蛋白质水平.
结论:
- 克鲁是40S和60S核糖体亚单元生物发生所需的.
- 在生物体内,Clu在全球蛋白质翻译中起着至关重要的作用.
- 克鲁具有多方面的作用,调节线粒体蛋白质合成和核糖体生物发生.
相关概念视频
Ribosomal RNA Synthesis
13.1K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
13.1K
Riboswitches
8.1K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.1K
Ribosome Profiling
3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K
piRNA - Piwi-interacting RNAs
6.8K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
6.8K
Transcriptional Regulation: Riboswitches
3
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
3
RNA Polymerase II Accessory Proteins
9.1K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
9.1K


