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对Pasteurella multocida菌株的抗定数感应和抗生物膜活动

Arul Dhayalan¹, Awadhesh Prajapati², Revanaiah Yogisharadhya³

¹ICAR-National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), Post Box No. 6450, Yelahanka, Bengaluru, 560064, Karnataka, India.

Microbial pathogenesis

|October 31, 2024

概括

十二种Pasteurella multocida菌株,特别是A血清组,表现出抗定数感应活性. 菌株NIVEDIPm9显示出显著的抗生物膜和C6-AHL降解能力,与金属酶蛋白相关.

科学领域:

微生物学微生物学
分子生物学分子生物学
生物化学生物化学

背景情况:

定数感应 (QS) 是一种细胞间通信机制,对细菌毒性至关重要.
帕斯泰雷拉多达 (Pasteurella multocida) 引起帕斯泰雷洛症,这是一个严重的牲畜疾病.
准QS可能是对抗细菌感染的新策略.

研究的目的:

为了选Pasteurella multocida菌株的抗定数感应活性.
研究活性菌株的抗生物膜和分子降解能力.
为了识别潜在的分子点,涉及到定数感应抑制.

主要方法:

对52种Pasteurella multocida菌株进行查,以检测它们对Chromobacterium violaceum的抗定数感应活性.
通过无细胞提取物评估抗生物膜活性和C6-N-乙烯基荷莫塞林乳 (AHL) 降解.
整个基因组测序和in silico分析,包括分子对接研究,确定蛋白质与AHL分子.

主要成果:

在52个Pasteurella multocida菌株中,有12个,所有都是A血清组,显示出反定数感应活性.
菌株NIVEDIPm9表现出最高的抑制区,对大肠杆菌有强大的抗生物膜活性,以及C6-AHL的度依赖性降解.
通过对NIVEDIPm9的基因组注释,发现了四种金属酶蛋白;MBL1和MBL3通过对接研究显示了与OH-C10-AHL的高结合亲和力.

结论:

不同的Pasteurella multocida菌株具有抗定数感应特性.
菌株NIVEDIPm9是一个有希望的候选人,用于开发针对巴氏菌病中的定数感应的抗病毒性策略.
在P. multocida中的金属酶蛋白可能起到调节定决数感应信号的作用.

关键词:

反生物膜活动活动.反定数感应的反定数感应.分子对接是分子对接.这种植物是Pasteurella multocida.

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