迪布甲酸破坏[Ca2+],活性氧物种,[pH],蛋白激酶和线粒体活动,损害精子功能
Seung Hyun Park1, Myung Chan Gye1
1Department of Life Science and Institute for Natural Sciences, Hanyang University, Seoul 04763, Republic of Korea.
Journal of environmental sciences (China)
|October 31, 2024
概括
双甲酸盐 (DBP) 通过破坏和pH水平,增加氧化应激,损害线粒体,损害精子功能. 虽然低水平的DBP可能会增强精子容量,但高剂量会严重降低运动能力和生育能力.
科学领域:
- 生殖毒理学 生殖毒理学
- 精子生物学 精子生物学
- 环境健康 环境健康
背景情况:
- 酸盐,如迪布酸 (DBP),是环境污染物,与生殖健康问题有关.
- 了解DBP诱导的精子功能障碍的具体机制对于评估其对男性生育能力的影响至关重要.
研究的目的:
- 阐明双甲 (DBP) 引起精子功能障碍的复杂机制.
- 研究DBP对各种精子参数的剂量依赖性影响,包括细胞内信号传递,氧化应激,线粒体功能和运动性.
主要方法:
- 将小鼠精子暴露在不同度的DBP (1-100μg/mL) 中.
- 细胞内 ([Ca2+]i) 和pH ([pH]i),活性氧物种 (ROS),脂质过氧化 (LPO),线粒体透性过渡孔 (mPTP) 开口,线粒体膜潜力 (MMP) 和腺三酸盐 (ATP) 水平的评估.
- 评价精子的运动性,自发的体反应,尾巴曲和蛋白质酸化 (PKA基质和胺蛋白质).
主要成果:
- DBP对细胞内pH值表现出双相影响,对精子参数产生剂量依赖的影响.
- 低DBP度 (1-10μg/mL) 增加了自发的体反应,并激活了PKA和氨酸酸化,表明精子容量增强.
- 高DBP度 (100μg/mL) 导致细胞内和ROS升高,LPO增加,线粒体功能受损 (MMP和ATP降低),精子运动和尾部完整性受损.
结论:
- DBP对精子生理学产生复杂,剂量依赖的影响.
- 在低的环境相关度下,DBP可以通过ROS介导的信号来增强精子电容.
- 在较高剂量下,DBP通过压倒细胞防御机制并损害线粒体功能和运动性,诱导显著的精子功能障碍和潜在的不孕症.
相关概念视频
Spermatogenesis
102.2K
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
102.2K
Teratogenicity
2.4K
The ability of a drug to produce structural deformations and functional abnormalities in the developing embryo or the fetus is called teratogenicity, and the drug producing this effect is known as a teratogen. Teratogenic effects include stillbirth, miscarriage, intrauterine growth restriction, and neurocognitive delay. A teratogen may affect the embryo at different stages of development, which is important in determining the type and extent of the damage. During blastocyst formation, the early...
2.4K
Factors Affecting Drug Distribution: Physiological Barriers
178
Drug distribution in the body is intricately regulated by various physiological barriers that control the passage of substances. These include the capillary endothelial barrier, the blood-brain, blood-cerebrospinal fluid, blood-placental, and blood-testis barriers.
The capillary endothelial barrier allows only smaller molecules below 600 Da (Daltons) to pass through. It also restricts drugs like heparin that are bound to blood components, limiting their movement within the bloodstream.
The...
The capillary endothelial barrier allows only smaller molecules below 600 Da (Daltons) to pass through. It also restricts drugs like heparin that are bound to blood components, limiting their movement within the bloodstream.
The...
178
Infertility in Males
250
Male infertility affects millions of couples worldwide, arising from various factors that impact different stages of the reproductive process. An endocrine imbalance resulting from conditions like hypogonadism, Klinefelter syndrome, or pituitary disorders can disrupt hormone levels and reduce sperm production. Testicular defects, such as tumors, cryptorchidism, atrophic testes, abnormal sperm morphology, and low sperm count or motility, may arise due to genetic factors, structural...
250
The Electron Transport Chain
16.2K
The electron transport chain or oxidative phosphorylation is an exothermic process in which free energy released during electron transfer reactions is coupled to ATP synthesis. This process is a significant source of energy in aerobic cells, and therefore inhibitors of the electron transport chain can be detrimental to the cell's metabolic processes.
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...
16.2K
pH Regulation in Cells
6.0K
pH plays a critical role in maintaining normal cellular activities. It helps maintain the structure and function of various proteins, dictates the charge on cellular membranes, and is crucial for metabolic reactions inside the cell. Moreover, cells use the energy from the proton motive force to generate ATP.
Cytosolic pH
Under physiological conditions, the cytosolic pH is slightly more acidic than the extracellular pH. However, cells must prevent further acidification of their cytosol to...
Cytosolic pH
Under physiological conditions, the cytosolic pH is slightly more acidic than the extracellular pH. However, cells must prevent further acidification of their cytosol to...
6.0K


