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Updated: Jun 9, 2025

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分子探针用于跟踪脂质滴滴膜动态

Lingxiu Kong1, Qingjie Bai1,2, Cuicui Li3,4,5,6

  • 1School of Pharmaceutical Sciences, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, Shandong, 250117, PR China.

Nature communications
|November 1, 2024
PubMed
概括

研究人员开发了一种新型分子探针LDM,用于可视化脂滴膜 (LD). 这一突破使得能够详细研究活细胞中的LD膜动态和蛋白相互作用.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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