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Updated: Jun 9, 2025

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Published on: May 23, 2018

Seq-Scope:重新利用Illumina测序流动细胞来实现高分辨率的空间转录学

Yongsung Kim¹, Weiqiu Cheng², Chun-Seok Cho¹

¹Department of Molecular & Integrative Physiology, University of Michigan Medical School, Ann Arbor, MI, USA.

Nature protocols

|November 1, 2024

概括

Seq-Scope通过重新利用Illumina测序进行高分辨率基因表达分析来增强空间转录学. 这种具有成本效益的方法为分子生物学和组织学研究提供了亚微米分辨率和快速结果.

科学领域:

基因组学就是基因组学.
分子生物学分子生物学
历史学历史学历史学

背景情况:

空间转录组学使得基因表达与空间上下文进行分析.
目前的方法在分辨率,成本和可扩展性方面存在局限性.

研究的目的:

介绍Seq-Scope,这是一个用于高分辨率空间转录组分析的新协议.
克服现有的空间转录技术的局限性.

主要方法:

将Illumina NovaSeq 6000平台重新用于空间转录组学.
组织切片准备,图书馆构建和计算分析的详细协议.
在亚微米分辨率下整合组织学成像与转录基因数据.

主要成果:

Seq-Scope实现了亚微米分辨率和高捕获效率.
该协议可实现快速的交付时间 (3天内) 和成本效益.
证明了与转录基因数据一起精确的基因病理学注释.

结论:

Seq-Scope为高分辨率的空间转录组分析提供了一个可扩展和负担得起的解决方案.
该方法可以适应各种生物组织.
它是分子生物学和组织学研究人员的宝贵工具.

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相关概念视频

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...