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相关概念视频

Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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相关实验视频

Updated: Jun 9, 2025

Identification of Circular RNAs using RNA Sequencing
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Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

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在各种绵羊组织中进行全面的圆形RNA分析.

Mohammad Reza Bakhtiarizade1, Maryam Heidari2, Amir Hossein Mohammadi Ghanatghestani3

  • 1Department of Animal and Poultry Science, College of Aburaihan, University of Tehran, Tehran, Iran. mrbakhtiari@ut.ac.ir.

Scientific reports
|November 1, 2024
PubMed
概括

这项研究在12个绵羊组织中绘制了循环RNA (circRNA) 表达的地图,确定了8,831个高度可靠的circRNA,其中包括88个新型. 组织特异性的circRNAs表明它们在调节生理功能中的作用.

关键词:
功能性丰富分析的分析.没有编码的RNA.在RNA-Seqq.组织特异性的组织特异性的组织.在 lncRNAs 中.

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Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood
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Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood

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Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species

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相关实验视频

Last Updated: Jun 9, 2025

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08:25

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Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species
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科学领域:

  • 基因组学和分子生物学
  • 动物科学动物科学
  • 生物信息学是一种生物信息学.

背景情况:

  • 循环RNAs (circRNAs) 是至关重要的,但在绵羊中没有得到充分的研究.
  • 之前的羊 circRNA 研究集中在有限的组织上,缺乏全面的表达概况.
  • 了解circRNA表达对于绵羊基因组学和兽医研究至关重要.

研究的目的:

  • 在12个不同的绵羊组织中全面识别和描述circRNA表达.
  • 为了研究羊 circRNAs 的特征和组织特异性.
  • 探索circRNAs在绵羊生理学的功能影响.

主要方法:

  • 统一分析了来自12只绵羊组织的61个公共RNA测序数据集.
  • 应用严格的计算管道用于circRNA识别和表征.
  • 进行了组织特异性,共同表达和功能丰富性分析.

主要成果:

  • 鉴定了8,919个高保证度的外子衍生的circRNAs,包括88个新型circRNAs.
  • 发现了3059个组织特异性circRNAs,其特异性高于线性RNAs.
  • 组织特异性circRNAs的宿主基因被丰富了与各自组织相关的功能,表明了调节作用.

结论:

  • 这项研究介绍了在羊群中首个全面的circRNA表达格局,包括新型组织.
  • 组织特异性的circRNAs在调节不同绵羊组织的生理活动方面发挥着重要作用.
  • 这些发现为绵羊基因组和兽医研究提供了宝贵的资源,突出了共同的监管机制.