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牛奶特征的表征和与DGAT1多形态的关联研究在巴格诺雷斯羊群中

Maria Giulia Pugliano¹, Gianfranco Cosenza², Emanuele D'Anza¹

¹Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Naples, Italy.

Animal bioscience

|November 1, 2024

概括

该研究在巴格诺雷斯羊中发现了一种特定的DGAT1基因变异 (CT基因型),该变异与乳脂和蛋白质产量增加有关,为繁殖计划提供了标记. 这项研究为改善本土羊品种提供了宝贵的遗传见解.

科学领域:

动物遗传学动物遗传学
乳制品科学乳制品科学
分子生物学分子生物学

背景情况:

巴格诺莱斯羊是一种来自意大利坎帕尼亚的双重用途本土品种,对Pecorino Bagnolese奶酪生产至关重要.
关于巴格诺莱羊的遗传数据有限,特别是影响牛奶生产特征的基因.
了解影响牛奶产量和成分的遗传因素对于品种改进和可持续乳制品养殖至关重要.

研究的目的:

为了研究巴格诺雷斯羊的牛奶生产特征.
分析该品种内的二甲酸甘油酸转移酶1 (DGAT1) 基因的遗传变异性.
确定DGAT1基因多态和牛奶生产特征之间的关联.

主要方法:

用Kjeldahl和Gerber方法收集牛奶样本,对牛奶产量,蛋白质和脂肪含量进行定量分析.
聚合酶链反应-限制片段长度多态 (PCR-RFLP) 用于在DGAT1位点 (g.5553C>T和g.8539C>T) 的特定单核酸多态 (SNP) 的基因型定型.
进行了统计关联分析,以将DGAT1基因型与观察到的牛奶生产特征联系起来.

主要成果:

巴格诺尔羊的牛奶含有高度的脂肪和蛋白质.
在DGAT1基因中观察到g.5553C (0.56) 和g.8539C (0.95) 的高等位基因频率.
与其他基因型相比,g.5553C>T SNP的CT基因型与较高的牛奶脂肪和蛋白质产量百分比显著相关 (p<0.01).

结论:

巴格诺雷斯羊的牛奶成分支持高质量的乳制品产量.
位于DGAT1 g.5553 SNP的CT基因型作为一种潜在的基因标记物,可以增强巴格诺羊的牛奶脂肪和蛋白质生产.
使用的基因型定型方法是成本效益高且可靠的,用于在绵羊群中表征DGAT1SNP.

关键词:

协会分析协会分析公共交通总局1 牛奶脂肪牛奶脂肪牛奶蛋白是一种牛奶蛋白.Ovis aries 的使用情况.单核酸多态 (单核酸多态) (简称单核酸多态)

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