Chi-circ_0009659通过miR-3431-5p/STEAP4轴调节山羊肌内脂肪细胞的分化
Xin Li1,2,3, Hao Zhang1,2,3, Yong Wang1,2,3
1Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education, Southwest Minzu University, Chengdu 610041 , China.
Animal bioscience
|November 1, 2024
概括
循环RNAs (circRNAs) 调节山羊肌内脂肪细胞的分化. Chi-circ_0009659通过海绵化miR-3431-5p促进分化,影响STEAP4的表达.
科学领域:
- 动物科学动物科学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 循环RNAs (circRNAs) 是动物脂质沉积的关键调节者.
- 在山羊的肌肉内脂肪细胞分化控制的circRNAs上存在有限的研究.
- 鉴定出Chi-circ_0009659在山羊脂肪细胞中表达高.
研究的目的:
- 为了研究chi-circ_0009659在山羊肌内脂肪细胞分化中的作用.
- 为了阐明涉及chi-circ_0009659,miR-3431-5p和STEAP4.4的分子机制.
主要方法:
- 定量PCR (qPCR) 检测在分化过程中的chi-circ_0009659表达.
- 腺病毒和智能消声器用于功能丧失和功能获取模型.
- 油红色O和Bodipy染色用于脂肪细胞分化评估.
- 光在位杂交 (FISH) 和核细胞质分离用于定位.
- 生物信息学,RNA拉下,和双光酶测试来验证相互作用.
主要成果:
- 沉默chi-circ_0009659抑制了脂质积累和基因表达的分化.
- 过度表达chi-circ_0009659促进了脂肪细胞的分化.
- 位于细胞质中的Chi-circ_0009659调节miR-3431-5p,从而影响到STEAP4.4.
- miR-3431-5p抑制了分化,而STEAP4则促进了分化.
结论:
- Chi-circ_0009659积极调节山羊肌肉前脂肪细胞的分化.
- 该机制涉及chi-circ_0009659海绵miR-3431-5p调节STEAP4表达的机制.
- 这项研究提供了关于脂肪细胞分化中的circRNA功能的见解.
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