解读肉的腹部脂肪中脂肪细胞分化的解读机制
Xi Sun1, Xiaoying Liu1, Chaohui Wang1
1College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
Journal of agricultural and food chemistry
|November 1, 2024
概括
这项研究揭示了在肉腹部脂肪组织发育过程中的关键分子和表观遗传变化. 它识别了参与脂肪细胞分化的关键基因和转录因子,为过度脂肪沉积提供了洞察力.
科学领域:
- 动物科学动物科学
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 在 brojlers 中过度的腹部脂肪组织 (AFT) 沉积是家禽行业的重大经济问题.
- 推动AFT发展的精确分子机制仍然不完全理解.
研究的目的:
- 阐明在肉AFT中的脂肪细胞分化过程中染色质可访问性和基因表达的动态变化.
- 为了确定关键的基因,途径和转录因子参与调节AFT发展.
主要方法:
- 结合RNA测序 (RNA-seq) 和转化酶可访问的染色体测序 (ATAC-seq) 以在不同发育阶段 (D3和D14) 描述AFT.
- 为了验证发现,利用了不朽化肉前脂肪细胞 (ICP2) 的体外模型.
- 分析了差异性基因表达,通路丰富,染色质可访问性变化和转录因子动机.
主要成果:
- 识别了关键脂肪生成标记物 (例如,C/EBPα,FABP4,PPARγ) 和细胞循环调节剂 (例如,CDK1,PCNA) 的差异表达.
- RNA-seq揭示了与脂肪细胞分化,DNA复制和细胞周期变化相关的途径.
- ATAC-seq确定了染色质可访问性的显著变化,涉及诸如甘氨酸脂代谢和TGF-β信号传递等途径,并预测了159个转录因子.
结论:
- 这项研究提供了一个全面的框架,以了解肉AFT中脂肪细胞分化的表观遗传调节.
- 确定了在AFT发展中发挥关键作用的候选基因和转录因子,为未来的研究和干预提供了潜在的目标.
相关概念视频
Lipid Digestion
Lipids are large molecules that are generally not water-soluble. Since most of the digestive enzymes in the human body are water-based, there are specific steps the body must take to break down lipids and make them available for use.
Protein Digestion
Protein digestion begins in the stomach, where the highly acidic environment can easily disrupt protein structure by exposing the peptide bonds of polypeptide chains. After polypeptide chains are broken into individual amino acids by a series of digestive enzymes, the amino acids are transported to the liver via the bloodstream to produce energy.
Overview of Fatty Acid Metabolism
Lipids also are sources of energy that power cellular processes. Like carbohydrates, lipids are composed of carbon, hydrogen, and oxygen, but these atoms are arranged differently. Most lipids are nonpolar and hydrophobic. Major types include fats and oils, waxes, phospholipids, and steroids.
Fatty acids are catabolized in a process called beta-oxidation, which takes place in the matrix of the mitochondria and converts their fatty acid chains into two-carbon units of acetyl groups. The acetyl...
Fatty acids are catabolized in a process called beta-oxidation, which takes place in the matrix of the mitochondria and converts their fatty acid chains into two-carbon units of acetyl groups. The acetyl...
Mechanical and Chemical Digestion in the Small Intestine
The small intestine plays a crucial role in our digestive system, performing both mechanical and chemical digestion.
Mechanical digestion in the small intestine involves movements such as segmentations and migrating motility complexes (MMCs), primarily controlled by the myenteric plexus. Segmentations are localized contractions occurring in areas of the intestine distended by chyme—a mixture of partially digested food. These contractions mix chyme with digestive juices, facilitating absorption...
Mechanical digestion in the small intestine involves movements such as segmentations and migrating motility complexes (MMCs), primarily controlled by the myenteric plexus. Segmentations are localized contractions occurring in areas of the intestine distended by chyme—a mixture of partially digested food. These contractions mix chyme with digestive juices, facilitating absorption...
Overview of Lipid Metabolism
Lipid metabolism is a crucial process in the human body that involves the synthesis and degradation of lipids. This process is essential for energy production, cell membrane formation, and hormone production, among other functions.
Lipolysis: The Breakdown of Lipids:
Lipolysis is the process of breaking down lipids, particularly triglycerides, into glycerol and fatty acids. This process typically occurs in the adipose tissue and is triggered by various hormones, including glucagon and...
Lipolysis: The Breakdown of Lipids:
Lipolysis is the process of breaking down lipids, particularly triglycerides, into glycerol and fatty acids. This process typically occurs in the adipose tissue and is triggered by various hormones, including glucagon and...
Lipid Absorption
Dietary triglycerides from chyme in the duodenum are mixed with bile salts produced by the liver to emulsify fats. As a result, large droplets are broken down into smaller ones, increasing the surface area for enzymatic action. Once emulsified, pancreatic lipases hydrolyze the triglycerides into free fatty acids and monoglycerides.
These breakdown products bind with bile salts and lecithin to form micelles, which quickly pass between microvilli to come in close contact with the apical...
These breakdown products bind with bile salts and lecithin to form micelles, which quickly pass between microvilli to come in close contact with the apical...


