多倍体癌细胞显示化学疗法耐药性的标志
James Hicks1, Michael Schmidt1, Amin Nahgdloo1
1University of Southern California.
Research square
|November 1, 2024
概括
识别为循环瘤细胞大颗粒集群 (CTC-IGCs) 的大型多类癌细胞与晚期前列腺癌的生存率较差有关. 像HOMER1这样的新型标记物与化疗耐药性和癌症复发有关.
科学领域:
- 在瘤学瘤学.
- 癌症生物学 癌症生物学
- 基因组学就是基因组学.
背景情况:
- 癌症的治疗耐药性导致患者死亡率和疾病复发.
- 大型多化癌细胞,特别是在晚期前列腺癌中,与疾病的进展和复发有关.
- 循环瘤细胞大颗粒群 (CTC-IGCs) 代表了晚期疾病的潜在标志物.
研究的目的:
- 研究CTC-IGCs在晚期前列腺癌患者的预后意义.
- 为了识别与多倍体癌细胞中的化疗耐药性相关的分子标记物.
- 探索多类癌细胞在治疗耐药性和癌症复发中的作用.
主要方法:
- 对44名晚期前列腺癌患者的骨髓吸血液进行分析.
- 单个单元格的副本编号对CTC-IGCs的概况.
- 单细胞DNA和RNA测序,以及用多化癌细胞 (PC3,MDA-MB-231) 诱导的蛋白质免疫光,这些癌细胞接受了多塞塔塞尔或西斯治疗.
主要成果:
- CTC-IGCs的存在与较差的无进展生存率有显著的关联.
- CTC-IGCs显示了克隆起源,表明完全的多化.
- 新型RNA和蛋白质标记物 (HOMER1,TNFRSF9,LRP1) 在耐化疗多体细胞和患者CTC中被发现,与公开数据中的复发相关.
结论:
- 大型多倍型瘤细胞,包括CTC-IGCs,在晚期前列腺癌中具有显著的预后价值.
- 这些多倍体细胞在化学疗法耐药性中起作用.
- 已识别的标记物 (HOMER1,TNFRSF9,LRP1) 提供了针对癌症复发的新治疗策略的潜力.
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