相关实验视频

Updated: Jun 8, 2025

Author Spotlight: Unveiling the Molecular Basis of Pain Perception and Neuropathic Pain

Author Spotlight: Unveiling the Molecular Basis of Pain Perception and Neuropathic Pain

Published on: August 9, 2024

TSH可以增强神经元外生长

Maryam Mansoori¹, Rauf Latif^1,2, Syed A Morshed^1,2

¹Thyroid Research Unit, Icahn School of Medicine at Mount Sinai, New York, NY, United States.

Frontiers in endocrinology

|November 1, 2024

概括

甲状腺刺激激素 (TSH) 通过激活TSH受体 (TSHR) 在分化的神经细胞中促进神经元外生. 这种信号通路影响神经元通信,可能对神经退行性疾病产生影响.

科学领域:

神经内分泌学神经内分泌学
细胞生物学细胞生物学
分子神经科学分子神经科学

背景情况:

甲状腺刺激激素 (TSH) 作用超越甲状腺,TSH受体 (TSHR) 在大脑区域表达.
TSH对神经元表型和神经元外生长的影响在很大程度上仍未被探索.

研究的目的:

用人类神经母细胞瘤细胞系研究TSH对神经元分化和神经元外生的影响.
描述特异化神经元细胞中TSHR表达的特征,并阐明TSH激活的下游信号通路.

主要方法:

在18天内,SH-SY5Y细胞的神经分化.
使用绝对数字PCR的TSHR mRNA和蛋白质的量化.
通过ImageJ分析评估神经元外生长.
细胞内信号通路 (cAMP,PI3K/AKT,NFkB,MAPK) 的分析.

主要成果:

不同化的SH-SY5Y细胞表达了高水平的TSHR mRNA和蛋白质.
暴露于TSH显著增加了初级,二级和三级神经元的生长,以剂量依赖的方式.
TSHR信号激活了cAMP,pPI3K,pAKT和pNFkB通路,同时抑制了pMAPK,与增强的神经元外生相关.

结论:

TSH/TSHR轴在促进神经元中神经元外生长方面发挥着作用.
这些发现表明,TSH在神经元发育和通信中的新功能.
需要进行进一步的研究,以探索TSH诱导的神经元生长在神经退行性疾病中的病理生理学意义.

关键词:

甲状腺刺激激素 (TSH) 是一种激素.甲状腺刺激激素受体 (TSHR) 是一种差异化协议的分化协议神经质长度神经质长度神经细胞外生长的神经细胞外生长

更多相关视频

Dissection and Culture of Chick Statoacoustic Ganglion and Spinal Cord Explants in Collagen Gels for Neurite Outgrowth Assays

Dissection and Culture of Chick Statoacoustic Ganglion and Spinal Cord Explants in Collagen Gels for Neurite Outgrowth Assays

Published on: December 20, 2011

An Enhanced Green Fluorescence Protein-based Assay for Studying Neurite Outgrowth in Primary Neurons

An Enhanced Green Fluorescence Protein-based Assay for Studying Neurite Outgrowth in Primary Neurons

Published on: October 19, 2019

相关概念视频

Enzyme-linked Receptors

Enzyme-linked Receptors

Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...

Synthesis and Regulation of Thyroid Hormones

Synthesis and Regulation of Thyroid Hormones

Low blood levels of the thyroid hormones — triiodothyronine (T3) and thyroxine (T4) — signal the hypothalamus to release the thyrotropin-releasing hormone (TRH). TRH then reaches the pituitary gland and stimulates the release of thyroid-stimulating hormone(TSH) into the bloodstream.
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The...

Neurogenesis and Regeneration of Nervous Tissue

Neurogenesis and Regeneration of Nervous Tissue

In the CNS, neurogenesis, the birth of new neurons from stem cells, is limited to the hippocampus in adults. In other regions of the brain and spinal cord, neurogenesis is almost non-existent due to inhibitory influences from neuroglia, especially oligodendrocytes, and the absence of growth-stimulating cues. The myelin produced by oligodendrocytes in the CNS inhibits neuronal regeneration. Furthermore, astrocytes proliferate rapidly after neuronal damage, forming scar tissue that physically...