相关实验视频

Updated: Jun 8, 2025

Author Spotlight: Unraveling the Dynamics of Eukaryotic DNA Replication Through Single-Molecule Visualization
07:37

Author Spotlight: Unraveling the Dynamics of Eukaryotic DNA Replication Through Single-Molecule Visualization

Published on: September 27, 2024

1.4K

一个高吞吐量单分子平台,用于研究DNA超卷对蛋白质-DNA相互作用的影响

Huijin Lee1,2, Jihee Hwang2,3, Fahad Rashid1

  • 1Department of Biophysics and Biophysical Chemistry, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205, USA.

bioRxiv : the preprint server for biology
|November 1, 2024
PubMed
概括

DNA超级卷对蛋白质相互作用产生影响. 我们的新方法揭示了负超线圈增加了CRISPR-Cas9的脱效应,而正和负超线圈都增强了MutS与不匹配DNA的结合.

更多相关视频

Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage06:51

Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage

Published on: May 6, 2020

3.9K
A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA12:05

A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA

Published on: October 1, 2017

8.1K

相关概念视频

Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
13.9K