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细胞和分子机制塑造了小鼠和鼠尾椎骨比例的发展和演变

Ceri J Weber¹, Alexander J Weitzel¹, Alexander Y Liu¹

¹Department of Cell and Developmental Biology, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.

bioRxiv : the preprint server for biology

|November 1, 2024

概括

脊椎长度差异是由生长软骨高度和细胞增殖驱动的,而不仅仅是软骨细胞大小. 尿性受体C (NPR3) 基因影响哺乳动物的骨比例.

科学领域:

发育生物学发展生物学
进化生物学进化生物学
骨生物学骨生物学

背景情况:

脊椎延长机制仍然不太清楚,特别是在长鹿等物种中发现的不成比例的长度.
动物的尾巴为研究脊椎生长和比例多样性提供了一个可操作的模型.
杰博斯表现出异常长的尾椎,影响了整体身体比例.

研究的目的:

为了研究差异性脊椎延伸背后的细胞和分子机制.
确定负责确定动物骨比例的遗传调节者.
探索尿素受体C (NPR3) 在骨生长调节中的作用.

主要方法:

脊椎生长软骨高度和蜂性在小鼠和 jerboas 的比较分析.
在不成比例地长的脊椎中对过度缩的冠状细胞的组织学检查.
鼠标和鹿尾椎的交叉RNA测序 (RNA-Seq). 鼠标和鹿尾椎的交叉RNA测序 (RNA-Seq). 鼠标和鹿尾椎的交叉RNA测序 (RNA-Seq). 鼠标和鹿尾椎的交叉RNA测序 (RNA-Seq). 鼠标和鹿尾椎的交叉RNA测序 (RNA-Seq). 鼠标和鹿尾椎的交叉RNA测序 (RNA-Seq).
在具有功能丧失突变的小鼠模型中对NPR3的功能分析.

主要成果:

脊椎的长度主要取决于生长软骨的高度和经历内分泌骨化细胞的数量.
缩性冠状细胞的大小仅在最长的Jerboa尾脊椎中是一个重要的因素.
差异基因表达分析揭示了轴性身份和内分泌延伸的关键调节者,包括NPR3.
在小鼠中,NPR3功能丧失导致尾部和四肢比例异常.

结论:

不同的脊椎延伸是由生长软骨动态和细胞增殖的变化控制的.
在哺乳动物中,NPR3在调节局部骨生长率和整体身体比例方面发挥着至关重要的作用.
这些发现为骨生长机制和脊椎形式的进化多样化提供了基本的见解.

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