不断增长的巨细胞通过皮诺细胞化调节高水平的溶液流量
Biniam M Tebeje1, Natalie W Thiex2, Joel A Swanson1
1Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109-5620.
bioRxiv : the preprint server for biology
|November 1, 2024
概括
增长因子刺激细胞通过皮诺细胞化吸收溶解物. 这项研究表明,皮诺细胞形成对细胞生长和营养吸收至关重要,由营养的可用性和mTOR信号调节.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生化学
背景情况:
- 增长因子通过pinocytosis刺激细胞中溶解物质的吸收.
- 皮诺细胞酶在细胞生长和营养获取中发挥作用.
研究的目的:
- 为了研究皮诺细胞在细胞增殖中的作用.
- 通过皮诺细胞化来测量溶解物流速率,以应对殖民地刺激因子-1 (CSF1).
主要方法:
- 鼠巨细胞被培养并用CSF1.1进行治疗.
- 测量了细胞增殖和溶解物流速率.
- 使用了pinocytosis和mTOR的抑制剂.
主要成果:
- 在CSF1依赖的生长过程中,巨细胞每小时内化其细胞体积的72%.
- 氨酸缺乏增加了宏皮诺细胞形成,但限制了蛋白质合成和生长.
- 皮诺细胞形成能力不足以支持生长的素需求.
- 溶酶体循环分泌小分子,mTOR抑制剂减少了生长和循环.
结论:
- 皮诺细胞体对细胞生长和营养吸收至关重要.
- 营养感应通过pinocytosis调节溶解物流,涉及mTOR信号传递.
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